Scholarlink

<p>Unraveling gene function is crucial for enhancing quality traits in commercially cultivated strawberry (<i>Fragaria</i> × <i>ananassa</i>) through biotechnology. However, such analysis is hindered by the complexity of the octoploid genome (2<i>n</i> = 8<i>x</i> = 56) of <i>F.</i> × <i>ananassa</i>. In this study, we leveraged the ancestral wild strawberry species <i>Fragaria vesca</i> as an experimental model and developed an <i>Agrobacterium tumefaciens</i>–based leaf infiltration (agroinfiltration) technique to elucidate the functions of genes and regulatory sequences in strawberry. We transiently expressed genes encoding transcriptional regulators that govern floral organ development in <i>F. vesca</i> leaf cells, allowing us to investigate the underlying regulatory mechanisms through <i>in planta</i> gene expression and transactivation assays. Our agroinfiltration method serves as a practical tool for studying the activities of genes controlling economically important traits in strawberry.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Agroinfiltration technique for elucidating the functions of strawberry genes in Fragaria vesca

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年7月1日

查看全文
  • Chonprakun Thagun,
  • Yutaka Kodama

摘要

Unraveling gene function is crucial for enhancing quality traits in commercially cultivated strawberry (Fragaria × ananassa) through biotechnology. However, such analysis is hindered by the complexity of the octoploid genome (2n = 8x = 56) of F. × ananassa. In this study, we leveraged the ancestral wild strawberry species Fragaria vesca as an experimental model and developed an Agrobacterium tumefaciens–based leaf infiltration (agroinfiltration) technique to elucidate the functions of genes and regulatory sequences in strawberry. We transiently expressed genes encoding transcriptional regulators that govern floral organ development in F. vesca leaf cells, allowing us to investigate the underlying regulatory mechanisms through in planta gene expression and transactivation assays. Our agroinfiltration method serves as a practical tool for studying the activities of genes controlling economically important traits in strawberry.