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<p>Chrysotile asbestos, a well-established carcinogen, is known to induce various malignant cancers, contributing to an estimated annual death toll of 107,000 individuals due to asbestos-related diseases. While cell and animal models play a crucial role in elucidating the carcinogenic mechanisms of asbestos, existing models are plagued by prolonged experimental durations. In this investigation, we established a novel NIH/3T3 cell model of asbestos-induced malignant transformation using 3D culture techniques, followed by the development of a corresponding mouse model via orthotopic transplantation. Subsequent administration of the HIF-1α inhibitor PX-478 to both models allowed for the assessment of model reliability through the observation of malignant phenotypes and associated protein alterations.</p>

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Dual phase modeling of Chrysotile carcinogenesis from 3D cell transformation to orthotopic tumors

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年1月13日

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  • Yanan Gao,
  • Wenke Yu,
  • Rui Li,
  • Qi Wang,
  • Min Zhang,
  • Yichen Zhong,
  • Hailing Xia,
  • Fangfang Zhang,
  • Lijin Zhu

摘要

Chrysotile asbestos, a well-established carcinogen, is known to induce various malignant cancers, contributing to an estimated annual death toll of 107,000 individuals due to asbestos-related diseases. While cell and animal models play a crucial role in elucidating the carcinogenic mechanisms of asbestos, existing models are plagued by prolonged experimental durations. In this investigation, we established a novel NIH/3T3 cell model of asbestos-induced malignant transformation using 3D culture techniques, followed by the development of a corresponding mouse model via orthotopic transplantation. Subsequent administration of the HIF-1α inhibitor PX-478 to both models allowed for the assessment of model reliability through the observation of malignant phenotypes and associated protein alterations.