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<p>MicroRNAs (miRNAs) have multiple functions that regulate gene expression in various species. Few studies have explored the effects of miRNAs on the pathogenesis of Alzheimer’s disease (AD); however, the potential neuroprotective effects of miRNAs on AD, particularly by targeting neuronal markers, remain unclear. In this study, we suggested potential neuroprotective roles for miR-937-3p in an in vitro AD model, which has not been extensively studied. Our biological analysis confirmed that miR-937-3p participated in neuronal protection and differentiation. We selected miR-937-3p as a novel candidate and identified Netrin1 (NTN1), an axon guidance regulator, as its target gene via qPCR analysis and luciferase assay. Additionally, FACS analysis revealed a reduction in apoptosis levels in Aβ-treated cells following treatment with the miR-937-3p-I. Western blot analysis showed that the expression of Mcl-1, an anti-apoptotic marker, increased with miR-937-3p-I treatment in an in vitro AD model. Interestingly, the levels of pro-caspase 7, pro-caspase 3, and pro-PARP, which are usually downregulated when their cleaved forms are upregulated, were found to increase with miR-937-3p-I treatment. The expression levels of neuronal markers such as NeuN, NFH, Tuj1, SYP, and MAP2 were enhanced by miR-937-3p-I treatment in the in vitro AD model. Therefore, miR-937-3p inhibition might play a therapeutic and neuroprotective role in AD by promoting <i>NTN1</i> expression and repressing the apoptotic pathway.</p>

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Therapeutic effects of miR-937-3p by targeting NTN1 expression and regulating apoptosis in an Aβ-induced neuronal cell death

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年7月2日

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  • Jiyun Choi,
  • Haewon Jeong,
  • Mahesh Ramalingam,
  • Jinsu Hwang,
  • Seong-Jin Kim,
  • Byeong C. Kim,
  • Han-Seong Jeong,
  • Sujeong Jang

摘要

MicroRNAs (miRNAs) have multiple functions that regulate gene expression in various species. Few studies have explored the effects of miRNAs on the pathogenesis of Alzheimer’s disease (AD); however, the potential neuroprotective effects of miRNAs on AD, particularly by targeting neuronal markers, remain unclear. In this study, we suggested potential neuroprotective roles for miR-937-3p in an in vitro AD model, which has not been extensively studied. Our biological analysis confirmed that miR-937-3p participated in neuronal protection and differentiation. We selected miR-937-3p as a novel candidate and identified Netrin1 (NTN1), an axon guidance regulator, as its target gene via qPCR analysis and luciferase assay. Additionally, FACS analysis revealed a reduction in apoptosis levels in Aβ-treated cells following treatment with the miR-937-3p-I. Western blot analysis showed that the expression of Mcl-1, an anti-apoptotic marker, increased with miR-937-3p-I treatment in an in vitro AD model. Interestingly, the levels of pro-caspase 7, pro-caspase 3, and pro-PARP, which are usually downregulated when their cleaved forms are upregulated, were found to increase with miR-937-3p-I treatment. The expression levels of neuronal markers such as NeuN, NFH, Tuj1, SYP, and MAP2 were enhanced by miR-937-3p-I treatment in the in vitro AD model. Therefore, miR-937-3p inhibition might play a therapeutic and neuroprotective role in AD by promoting NTN1 expression and repressing the apoptotic pathway.