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<p>One of the most pressing issues in modern biomedicine is the search for new antimicrobial agents – antibiotics, peptides, bacteriolytic enzymes. This study, using transcriptomic and proteomic approaches, identified a new extracellular bacteriolytic enzyme of <i>Lysobacter capsici</i> XL1 – the amidase Ami. The enzyme was isolated and characterized. Ami was found to hydrolyze the amide bond between the carbohydrate and peptide fragments in bacterial peptidoglycans of chemotypes A1γ, A3α, and A4α. Ami lysed live target cells of opportunistic bacteria <i>Micrococcus luteus</i> Ac-2230<sup>T</sup>, <i>Bacillus cereus</i> 217, <i>Staphylococcus aureus</i> 209P, <i>Enterococcus faecium</i> FS86, phytopathogenic bacteria <i>Bacillus megaterium</i> MS941, <i>Curtobacterium flaccumfaciens</i> pv. <i>flaccumfaciens</i>, and pathogenic bacteria of various strains of <i>B. anthracis</i>, including plasmid strains 71/12 and ΔAmes, as well as strains of <i>B. cereus</i> with hemolytic, lecithinase, and phosphatase activities. Thus, the bacteriolytic amidase Ami is a promising candidate for the development of next-generation antimicrobial drugs.</p>

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A new bacteriolytic amidase Ami of Lysobacter capsici XL1

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年7月1日

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  • Irina Kudryakova,
  • Alexey Afoshin,
  • Sergey Tarlachkov,
  • Sofya Pavlenko,
  • Natalia Suzina,
  • Nina Shishkova,
  • Elena Leontyevskaya,
  • Natalia Leontyevskaya

摘要

One of the most pressing issues in modern biomedicine is the search for new antimicrobial agents – antibiotics, peptides, bacteriolytic enzymes. This study, using transcriptomic and proteomic approaches, identified a new extracellular bacteriolytic enzyme of Lysobacter capsici XL1 – the amidase Ami. The enzyme was isolated and characterized. Ami was found to hydrolyze the amide bond between the carbohydrate and peptide fragments in bacterial peptidoglycans of chemotypes A1γ, A3α, and A4α. Ami lysed live target cells of opportunistic bacteria Micrococcus luteus Ac-2230T, Bacillus cereus 217, Staphylococcus aureus 209P, Enterococcus faecium FS86, phytopathogenic bacteria Bacillus megaterium MS941, Curtobacterium flaccumfaciens pv. flaccumfaciens, and pathogenic bacteria of various strains of B. anthracis, including plasmid strains 71/12 and ΔAmes, as well as strains of B. cereus with hemolytic, lecithinase, and phosphatase activities. Thus, the bacteriolytic amidase Ami is a promising candidate for the development of next-generation antimicrobial drugs.