Scholarlink

<p>This study aimed to analyze the antibiotic resistance patterns and virulence profiles of <i>Klebsiella pneumoniae</i>, a prevalent gram-negative pathogen linked to CLABSI patients globally. Of a total of 185 microbial isolates, 51 (27.5%) were <i>K</i>. <i>pneumoniae</i> isolates. The results of antimicrobial susceptibility testing using the disk diffusion method were compared to those of the VITEK-2. Phenotypic analysis revealed that 88.3% were biofilm producers and 50.9% were extended-spectrum beta-lactamase (ESBL) producers. Enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) revealed a discriminatory power of 0.7111 between ten selected isolates. The PCR detection of the virulence genes, including <i>FimH</i>, <i>rmpA</i>, <i>iutA</i>, and <i>fyuA</i>, revealed that the ten selected isolates harbored all these genes, except one without the <i>fyuA</i> gene. The presence of the <i>rmpA</i> and the <i>iutA</i> genes confirmed them as hypervirulent (hv) <i>K</i>. <i>pneumoniae</i>. The genes (<i>EAST-1</i>, <i>CNF-1</i>) were present in 20% and 60% of the isolates, respectively. All isolates had the <i>bla</i><sub><i>TEM</i></sub> and <i>bla</i><sub><i>SHV</i></sub> resistance genes, while 50% had the <i>bla</i><sub><i>NDM</i></sub> carbapenemase resistance gene. In conclusion, all selected <i>K</i>. <i>pneumoniae</i> isolates were proven to be ESBL producers and carbapenem-resistant, highlighting significant multidrug resistance. We found a strong correlation between biofilm formation and multidrug resistance, as well as between phenotypic and genotypic detection of various virulence factors. Infections from hyKp strains represent a growing public threat. Our findings aim to enhance therapeutic options for patients and help reduce complications and mortality.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Exploring virulence factors, virulome, and multidrug resistance of Klebsiella pneumoniae strains isolated from patients with central Line-associated bloodstream infections

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年6月20日

查看全文
  • Mohab G. Sayed,
  • Moselhy S. Mansy,
  • Mervat I. El Borhamy,
  • Heba M. Elsherif

摘要

This study aimed to analyze the antibiotic resistance patterns and virulence profiles of Klebsiella pneumoniae, a prevalent gram-negative pathogen linked to CLABSI patients globally. Of a total of 185 microbial isolates, 51 (27.5%) were K. pneumoniae isolates. The results of antimicrobial susceptibility testing using the disk diffusion method were compared to those of the VITEK-2. Phenotypic analysis revealed that 88.3% were biofilm producers and 50.9% were extended-spectrum beta-lactamase (ESBL) producers. Enterobacterial repetitive intergenic consensus-polymerase chain reaction (ERIC-PCR) revealed a discriminatory power of 0.7111 between ten selected isolates. The PCR detection of the virulence genes, including FimH, rmpA, iutA, and fyuA, revealed that the ten selected isolates harbored all these genes, except one without the fyuA gene. The presence of the rmpA and the iutA genes confirmed them as hypervirulent (hv) K. pneumoniae. The genes (EAST-1, CNF-1) were present in 20% and 60% of the isolates, respectively. All isolates had the blaTEM and blaSHV resistance genes, while 50% had the blaNDM carbapenemase resistance gene. In conclusion, all selected K. pneumoniae isolates were proven to be ESBL producers and carbapenem-resistant, highlighting significant multidrug resistance. We found a strong correlation between biofilm formation and multidrug resistance, as well as between phenotypic and genotypic detection of various virulence factors. Infections from hyKp strains represent a growing public threat. Our findings aim to enhance therapeutic options for patients and help reduce complications and mortality.