<p>Breast cancer (BC) has become a severe threat to women, which hasimposed excessive pressure on society. LncRNAs play a crucial role in the occurrenceand development of BC. This study aimed to evaluate the lncRNA SIAH2 antisense RNA 1(SIAH2-AS1) role in the development and progression of BC and explore the mechanismof SIAH2-AS1 related Wnt signaling pathway in BC. Malignant and paracancer normalbreast tissue samples were obtained from patients who underwent surgery at theSecond Affiliated Hospital of Zunyi Medical University. Subsequently, quantitativeRT-PCR (qRT-PCR) was performed with these acquired tissue samples to evaluate theconcentrations of SIAH2-AS1. Furthermore, CCK-8 assays, colony formation, woundhealing, and transwell were performed to investigate the cell proliferation,migration, and invasion respectively. Western blotting was eventually performed forthe investigation of proteins and EMT-related markers in the Wnt/β-catenin signalingpathway. The expression of SIAH2-AS1 was up-regulated in cancer tissues and cells.Cell proliferation, colony formation, invasiveness, and migration are significantlyreduced by silencing SIAH2-AS1. Moreover, E-cadherin expression in BC cells wasincreased, whereas N-cadherin and vimentin expression was decreased, when SIAH2-AS1was eliminated from the cells. Additionally, the Wnt/β-catenin signaling pathwayscyclin D1 and C-myc proteins were also significantly downregulated in BC cells whenSIAH2-AS1 was knocked out. Our study confirms that SIAH2-AS1 activates theWnt/β-catenin pathway and has an oncogenic activity that promotes the prognosis ofBC, suggesting that SIAH2-AS1 may be a potential drug target for BC. The developmentof small - molecule inhibitors capable of specifically targeting SIAH2 - AS1 totarget and modify the SIAH2 - AS1 gene in cancer cells may offer a novel strategyfor clinical treatment. Keywords: breast cancer; cell proliferation; cell migration;Long noncoding RNA; Wnt signaling pathway</p>