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<p>Bio-functionalized chalcogenide infrared optical glass fibers have been designed for evanescent wave mid-infrared spectroscopy. Surface biotinylation of the fiber tapered sensing zone has been achieved by reactivity of a maleimide function on sulfhydryl moieties of the glassy surface. Biotin-streptavidin interactions were studied by fiber evanescent wave spectroscopy. Kinetic measurement comparisons of functionalized and non-functionalized fiber surfaces for various protein concentrations have demonstrated the efficient bio-selectivity of the functionalized glass fibers. The protein enrichment of the functionalized glassy surface allows for a significant increase of the protein detection limit, greater than two orders of magnitude as compared to reference non-functionalized fibers. A detection of minute quantities at concentrations as low as 10 parts-per-billion is demonstrated. This study shows that bio-functionalized chalcogenide optical fibers allow to combine successfully surface bio-selectivity and infrared absorption fingerprints measurements to get a remarkable sensitivity enhancement of fiber evanescent wave detection methods in the mid infrared spectral range.</p>

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Biofunctionalization of chalcogenide glass fiber to enhance real time and label free detection by mid infrared spectroscopy

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年7月1日

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  • Rayan Zaiter,
  • Frédéric Adamietz,
  • Frédéric Désévédavy,
  • Clément Strutynski,
  • Damien Bailleul,
  • Frédéric Smektala,
  • Thierry Buffeteau,
  • Luc Vellutini,
  • Marc Dussauze

摘要

Bio-functionalized chalcogenide infrared optical glass fibers have been designed for evanescent wave mid-infrared spectroscopy. Surface biotinylation of the fiber tapered sensing zone has been achieved by reactivity of a maleimide function on sulfhydryl moieties of the glassy surface. Biotin-streptavidin interactions were studied by fiber evanescent wave spectroscopy. Kinetic measurement comparisons of functionalized and non-functionalized fiber surfaces for various protein concentrations have demonstrated the efficient bio-selectivity of the functionalized glass fibers. The protein enrichment of the functionalized glassy surface allows for a significant increase of the protein detection limit, greater than two orders of magnitude as compared to reference non-functionalized fibers. A detection of minute quantities at concentrations as low as 10 parts-per-billion is demonstrated. This study shows that bio-functionalized chalcogenide optical fibers allow to combine successfully surface bio-selectivity and infrared absorption fingerprints measurements to get a remarkable sensitivity enhancement of fiber evanescent wave detection methods in the mid infrared spectral range.