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<p><i>Bradyrhizobium</i> sp. DOA9 can nodulate a wide spectrum of legumes; however, unlike other bradyrhizobia, DOA9 carries a symbiotic plasmid harboring type III secretion system (T3SS) and several effector (T3E) genes, one of which encodes a putative type III effector SkP48. Here, we demonstrated the pivotal roles of SkP48 from <i>Bradyrhizobium</i> sp. DOA9 in inhibiting nodulation of various <i>Vigna</i> species and <i>Crotalaria juncea</i> and suppressing nodulation efficiency of <i>Arachis hypogaea</i>. By contrast, the nodulation efficiency of a SkP48 mutant did not differ significantly with the DOA9 wild-type strain on <i>Macroptilium atropurpureum</i> and <i>Stylosanthes hamata</i>. The SUMO domain of SkP48 is primarily responsible for the blocking nodulation phenotype <i>V. radiata</i>. An evolutionary analysis revealed that the SkP48 which contains a shikimate kinase and a SUMO protease (C48 cysteine peptidase) domain, SkP48 is distinct from other effectors previously reported in other bradyrhizobia and pathogenic bacteria. Our findings suggest that the putative T3E SkP48 is a key factor suppressing nodulation and nodule organogenesis in several legumes by activation of effector-triggered immunity through salicylic acid biosynthesis induction, which is deleterious to rhizobial infection. In addition, nodulation may be modulated by the function of defensins involved in jasmonic acid signalling in <i>V. radiata</i> SUT1.</p>

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Putative type III effector SkP48 of Bradyrhizobium sp. DOA9 encoding a SUMO protease blocks nodulation in Vigna radiata

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年6月5日

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  • Pongdet Piromyou,
  • Pongpan Songwattana,
  • Jenjira Wongdee,
  • Teerana Greetatorn,
  • Pakpoom Boonchuen,
  • Tarnee Phimphong,
  • Hien P. Nguyen,
  • Monchai Manassila,
  • Waraporn Tanthanuch,
  • Bussayarat Maikhunthod,
  • Kamonluck Teamtisong,
  • Panlada Tittabutr,
  • Nantakorn Boonkerd,
  • Eric Giraud,
  • Neung Teaumroong

摘要

Bradyrhizobium sp. DOA9 can nodulate a wide spectrum of legumes; however, unlike other bradyrhizobia, DOA9 carries a symbiotic plasmid harboring type III secretion system (T3SS) and several effector (T3E) genes, one of which encodes a putative type III effector SkP48. Here, we demonstrated the pivotal roles of SkP48 from Bradyrhizobium sp. DOA9 in inhibiting nodulation of various Vigna species and Crotalaria juncea and suppressing nodulation efficiency of Arachis hypogaea. By contrast, the nodulation efficiency of a SkP48 mutant did not differ significantly with the DOA9 wild-type strain on Macroptilium atropurpureum and Stylosanthes hamata. The SUMO domain of SkP48 is primarily responsible for the blocking nodulation phenotype V. radiata. An evolutionary analysis revealed that the SkP48 which contains a shikimate kinase and a SUMO protease (C48 cysteine peptidase) domain, SkP48 is distinct from other effectors previously reported in other bradyrhizobia and pathogenic bacteria. Our findings suggest that the putative T3E SkP48 is a key factor suppressing nodulation and nodule organogenesis in several legumes by activation of effector-triggered immunity through salicylic acid biosynthesis induction, which is deleterious to rhizobial infection. In addition, nodulation may be modulated by the function of defensins involved in jasmonic acid signalling in V. radiata SUT1.