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<p>Traumatic brain injury (TBI) is a significant health challenge worldwide, with current diagnostics and treatment falling short due to the complex pathophysiology involved. Extracellular vesicles (EVs) play a crucial role in brain injury response and are promising biomarkers for understanding the progression of TBI. A 3D in vitro human brain tissue model, comprising neurons, astrocytes, and microglia was utilized to simulate TBI and investigate EV responses. EVs were isolated at multiple acute timepoints post-injury and microRNA (miRNA) profiling revealed transient dysregulation of several miRNAs that aligned with clinical and in vivo studies. Pathway analysis revealed that these miRNAs are associated with the phosphoinositide 3-kinase / protein kinase B (PI3K / AKT) cell signaling pathway, a key regulator of neuroprotection, cell survival and injury response in TBI. The data suggest that temporal dysregulation of miRNAs plays a critical role in driving cellular responses following tissue injury and may serve as an initial snapshot of signaling following TBI, informing future investigations into long-term injury progression. Additionally, these findings demonstrate the utility of using an in vitro brain tissue model to study EVs in TBI to help identify potential biomarkers for clinical utility.</p>

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Extracellular vesicles as biomarkers for traumatic brain injury using a 3D in vitro human brain tissue model

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年7月24日

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  • Peter Hsi,
  • Vishal Tandon,
  • David L. Kaplan

摘要

Traumatic brain injury (TBI) is a significant health challenge worldwide, with current diagnostics and treatment falling short due to the complex pathophysiology involved. Extracellular vesicles (EVs) play a crucial role in brain injury response and are promising biomarkers for understanding the progression of TBI. A 3D in vitro human brain tissue model, comprising neurons, astrocytes, and microglia was utilized to simulate TBI and investigate EV responses. EVs were isolated at multiple acute timepoints post-injury and microRNA (miRNA) profiling revealed transient dysregulation of several miRNAs that aligned with clinical and in vivo studies. Pathway analysis revealed that these miRNAs are associated with the phosphoinositide 3-kinase / protein kinase B (PI3K / AKT) cell signaling pathway, a key regulator of neuroprotection, cell survival and injury response in TBI. The data suggest that temporal dysregulation of miRNAs plays a critical role in driving cellular responses following tissue injury and may serve as an initial snapshot of signaling following TBI, informing future investigations into long-term injury progression. Additionally, these findings demonstrate the utility of using an in vitro brain tissue model to study EVs in TBI to help identify potential biomarkers for clinical utility.