Scholarlink

<p>Advances in nanoscience and nanotechnology have facilitated the application of nanoparticles in various biomedical fields. This study synthesized iron oxide/palladium nanoparticles modified with carbon quantum dots <b>(</b>Pd@CQD@Fe₃O₄<b>)</b> and evaluated their cytotoxic and anticancer effects on SK-OV-3 (ovarian carcinoma) and MCF-7 (breast adenocarcinoma) human cell lines. Characterization of Pd@CQD@Fe₃O₄ nanoparticles was performed using SEM, EDS, XPS, VSM and zeta potential analysis. The Pd@CQD@Fe₃O₄-treated group exhibited a significantly increased generation of reactive oxygen species (ROS) compared to the control group. To assess the cytotoxic activities of Pd@CQD@Fe₃O₄ nanoparticles, the MTT assay was utilized, revealing a dose- and time-dependent response. Flow cytometry-based apoptosis assays revealed that cytotoxicity was mediated through apoptosis in both MCF-7 and SK-OV-3 cells. Gene expression analysis utilizing real-time PCR revealed significant alterations in <i>Bax</i>, <i>Bcl-2</i>, <i>caspase-8</i>, and <i>caspase-9</i> levels following Pd@CQD@Fe₃O₄ treatment. Notably, the Pd@CQD@Fe₃O₄ nanoparticle exhibited higher cytotoxicity against SK-OV-3, MCF-7 cancerous cells compared to normal cells. Caspase-8 mRNA expression increased by 1.40-fold in MCF-7 and 1.11-fold in SK-OV-3 cell lines. Caspase-3/7 protein levels rose by 3.00-fold in Pd@CQD@Fe₃O₄-treated SK-OV-3 cells. Pd@CQD@Fe₃O₄ induced total apoptosis in 79.8% of MCF-7 and 77.01% of SK-OV-3 cells, as confirmed by flow cytometry, indicating strong pro-apoptotic activity in both cell lines. These findings suggest that Pd@CQD@Fe₃O₄ nanoparticles possess potent anticancer activity and offer the potential for targeted drug delivery enhancement.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Preparation of Iron oxide/palladium nanoparticles modified with carbon quantum dots (Pd@CQD@Fe3O4): insight to anti-cancer effect and ROS generation

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年5月19日

查看全文
  • Parnian Rajabzadeh,
  • Pooneh Zoorazma,
  • Maryam Abbasi,
  • Seyed Ataollah Sadat Shandiz

摘要

Advances in nanoscience and nanotechnology have facilitated the application of nanoparticles in various biomedical fields. This study synthesized iron oxide/palladium nanoparticles modified with carbon quantum dots (Pd@CQD@Fe₃O₄) and evaluated their cytotoxic and anticancer effects on SK-OV-3 (ovarian carcinoma) and MCF-7 (breast adenocarcinoma) human cell lines. Characterization of Pd@CQD@Fe₃O₄ nanoparticles was performed using SEM, EDS, XPS, VSM and zeta potential analysis. The Pd@CQD@Fe₃O₄-treated group exhibited a significantly increased generation of reactive oxygen species (ROS) compared to the control group. To assess the cytotoxic activities of Pd@CQD@Fe₃O₄ nanoparticles, the MTT assay was utilized, revealing a dose- and time-dependent response. Flow cytometry-based apoptosis assays revealed that cytotoxicity was mediated through apoptosis in both MCF-7 and SK-OV-3 cells. Gene expression analysis utilizing real-time PCR revealed significant alterations in Bax, Bcl-2, caspase-8, and caspase-9 levels following Pd@CQD@Fe₃O₄ treatment. Notably, the Pd@CQD@Fe₃O₄ nanoparticle exhibited higher cytotoxicity against SK-OV-3, MCF-7 cancerous cells compared to normal cells. Caspase-8 mRNA expression increased by 1.40-fold in MCF-7 and 1.11-fold in SK-OV-3 cell lines. Caspase-3/7 protein levels rose by 3.00-fold in Pd@CQD@Fe₃O₄-treated SK-OV-3 cells. Pd@CQD@Fe₃O₄ induced total apoptosis in 79.8% of MCF-7 and 77.01% of SK-OV-3 cells, as confirmed by flow cytometry, indicating strong pro-apoptotic activity in both cell lines. These findings suggest that Pd@CQD@Fe₃O₄ nanoparticles possess potent anticancer activity and offer the potential for targeted drug delivery enhancement.