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<p>Stored RNA plays a key role in seed germination, especially after seeds long-term storage. This study aimed to assess the correlation between germination potential (GP) and RNA integrity in common bean (<i>Phaseolus vulgaris</i> L.) seed accessions stored at -18&#xa0;°C for 19.25 to 43.75 years and in seeds subjected to artificial aging at high temperature (42&#xa0;°C) and relative humidity (100%). The GP of long-term preserved and artificially aged seed lots ranged from 3 to 100% and 38–87%, respectively. RNA integrity was evaluated using the RNA Integrity Number (RIN, Agilent Bioanalyzer software). RIN data suggested that: (1) Sample sizes for RIN analysis may need to vary based on the GP of the seeds; (2) RIN has the potential to predict physiological quality, especially in seeds with high GP; (3) RIN values by itself may not accurately reflect the rate of RNA degradation over time, necessitating comparison with a control to determine ΔRIN (the difference between the RIN values of the sample and the control); (4) ΔRIN has a significant positive correlation with GP. These findings highlight the potential of RNA integrity in seeds as a molecular marker for developing tests that complement germination tests.</p>

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Long-term preserved bean seeds exhibit high RNA integrity and high germination potential

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年4月11日

查看全文
  • Alisson Ferreira Dantas,
  • Tayara Colins Nunes,
  • Priscila Grynberg,
  • Ana Cristina Miranda Brasileiro,
  • Antonieta Nassif Salomão,
  • Solange Carvalho Barrios Roveri José,
  • Rosana Pereira Vianello,
  • Juliano Gomes Pádua,
  • Marília de Castro Rodrigues Pappas,
  • Marcos Aparecido Gimenes

摘要

Stored RNA plays a key role in seed germination, especially after seeds long-term storage. This study aimed to assess the correlation between germination potential (GP) and RNA integrity in common bean (Phaseolus vulgaris L.) seed accessions stored at -18 °C for 19.25 to 43.75 years and in seeds subjected to artificial aging at high temperature (42 °C) and relative humidity (100%). The GP of long-term preserved and artificially aged seed lots ranged from 3 to 100% and 38–87%, respectively. RNA integrity was evaluated using the RNA Integrity Number (RIN, Agilent Bioanalyzer software). RIN data suggested that: (1) Sample sizes for RIN analysis may need to vary based on the GP of the seeds; (2) RIN has the potential to predict physiological quality, especially in seeds with high GP; (3) RIN values by itself may not accurately reflect the rate of RNA degradation over time, necessitating comparison with a control to determine ΔRIN (the difference between the RIN values of the sample and the control); (4) ΔRIN has a significant positive correlation with GP. These findings highlight the potential of RNA integrity in seeds as a molecular marker for developing tests that complement germination tests.