Scholarlink

<p>The Asian citrus psyllid, <i>Diaphorina citri</i> Kuwayama (Hemiptera: Liviidae), is a major pest of global citriculture. In the Americas and in Asia, <i>D. citri</i> vectors the phloem-limited bacterium, <i>Candidatus</i> Liberibacter asiaticus (<i>C</i>Las), which causes the fatal citrus disease huanglongbing, or citrus greening. Cell lines derived from <i>D. citri</i> can provide insight into both the basic biology of this pest and <i>D. citri</i>-associated pathogens including <i>C</i>Las. We previously identified CLG#2 as the optimal medium for long-term growth of <i>D. citri</i> primary cell cultures. Here we report on the establishment and characterization of three continuous <i>D. citri</i> cell lines, Dici1, Dici3, and Dici5, that have been passaged for &gt; 40 times. Based on morphological and transcriptomic data, the Dici1 and Dici3 cell lines include undifferentiated and neurogenic progenitor cells. Dici1 and Dici5 are infected with <i>Wolbachia</i>. Both Dici1 and Dici5 are infected with <i>D. citri reovirus,</i> and Dici5 is also infected with <i>D. citri-associated C virus</i>. Dici3 is free of both <i>Wolbachia</i> and virus infection. These cell lines provide an ideal platform for the study of inter-microbial relationships as well as microbe interaction with host insect cells.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Continuous cell lines derived from the Asian citrus psyllid, Diaphorina citri, harbor viruses and Wolbachia

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年1月2日

查看全文
  • Ke Wu,
  • Emily D. Vu,
  • Saptarshi Ghosh,
  • Ruchir Mishra,
  • Bryony C. Bonning

摘要

The Asian citrus psyllid, Diaphorina citri Kuwayama (Hemiptera: Liviidae), is a major pest of global citriculture. In the Americas and in Asia, D. citri vectors the phloem-limited bacterium, Candidatus Liberibacter asiaticus (CLas), which causes the fatal citrus disease huanglongbing, or citrus greening. Cell lines derived from D. citri can provide insight into both the basic biology of this pest and D. citri-associated pathogens including CLas. We previously identified CLG#2 as the optimal medium for long-term growth of D. citri primary cell cultures. Here we report on the establishment and characterization of three continuous D. citri cell lines, Dici1, Dici3, and Dici5, that have been passaged for > 40 times. Based on morphological and transcriptomic data, the Dici1 and Dici3 cell lines include undifferentiated and neurogenic progenitor cells. Dici1 and Dici5 are infected with Wolbachia. Both Dici1 and Dici5 are infected with D. citri reovirus, and Dici5 is also infected with D. citri-associated C virus. Dici3 is free of both Wolbachia and virus infection. These cell lines provide an ideal platform for the study of inter-microbial relationships as well as microbe interaction with host insect cells.