Comparison of microwave and pulsed electric field methods on extracting antioxidant compounds from Arvaneh plant (Hymenocrater platystegius Rech. F)

Improving the quality of extracts derived from medicinal plants is a critical concern due to their extensive use across various industries. The Arvaneh plant (Hymenocrater platystegius Rech. F), a species native to the Khorasan province in Iran and belonging to the mint family, was the focus of this study. We optimized the extraction of Arvaneh plant using both microwave (MW) and pulsed electric field (PEF) techniques. The MW method was tested at different processing times (90, 180, and 270 s) and power levels (180, 540, and 900 W), while the PEF method was evaluated with varying electric field intensities (0.25, 3.25, and 6.25 kV/cm) and pulse numbers (10, 45, and 80) using a central composite design (CCD). The results revealed that extraction efficiency, total phenolic content, and total flavonoid content were significantly higher with the PEF method compared to the MW method (p < 0.05). Moreover, the PEF technique showed superior performance in preserving the antioxidant properties of the extract, as assessed by DPPH and FRAP methods. GC/MS analysis confirmed the presence of 27 bioactive compounds in the Arvaneh extract obtained through PEF-assisted extraction. In conclusion, the PEF method proved to be highly efficient for extracting bioactive compounds from the Arvaneh plant.