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<p>The leafhopper <i>Hishimonus sellatus</i> (Hemiptera: Cicadellidae) is a pervasive agricultural pest and a primary vector for phytoplasmas, including those causing mulberry dwarf and jujube witches’ broom diseases. Despite its economic significance and role in pathogen transmission, the lack of a reference genome has limited molecular research into its vector competence and biology. We generated a chromosome-level genome assembly for <i>H. sellatus</i> using a combination of long-read sequencing and Hi-C chromatin interaction data. The final assembly spans 1.38 Gb of which six chromosome-length scaffolds were assigned to Hi-C linkage groups and 14 shorter scaffolds remained unplaced. The assembly exhibits high contiguity, characterized by a scaffold N50 of 246.95 Mb. EarlGrey-based repeat annotation identified 794.50 Mb of masked sequence, corresponding to 57.57% of the genome. Evaluation of the gene space using the Hemiptera dataset (BUSCO) revealed a completeness score of 94.3%. We predicted a total of 31,761 protein-coding genes, with 98.04% (31,138 genes) receiving functional annotations from public databases. This genomic resource provides a foundational dataset for investigating the mechanisms of phytoplasma transmission, comparative genomics within the Deltocephalinae subfamily, and the development of targeted pest management strategies.</p>

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A chromosome-level genome assembly of the mulberry leafhopper Hishimonus sellatus, a vector of phytoplasma diseases

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年7月31日

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  • Yue-Mei Li,
  • Jing Song,
  • Wei-An Deng,
  • De-Long Guan,
  • Xiao-Dong Li

摘要

The leafhopper Hishimonus sellatus (Hemiptera: Cicadellidae) is a pervasive agricultural pest and a primary vector for phytoplasmas, including those causing mulberry dwarf and jujube witches’ broom diseases. Despite its economic significance and role in pathogen transmission, the lack of a reference genome has limited molecular research into its vector competence and biology. We generated a chromosome-level genome assembly for H. sellatus using a combination of long-read sequencing and Hi-C chromatin interaction data. The final assembly spans 1.38 Gb of which six chromosome-length scaffolds were assigned to Hi-C linkage groups and 14 shorter scaffolds remained unplaced. The assembly exhibits high contiguity, characterized by a scaffold N50 of 246.95 Mb. EarlGrey-based repeat annotation identified 794.50 Mb of masked sequence, corresponding to 57.57% of the genome. Evaluation of the gene space using the Hemiptera dataset (BUSCO) revealed a completeness score of 94.3%. We predicted a total of 31,761 protein-coding genes, with 98.04% (31,138 genes) receiving functional annotations from public databases. This genomic resource provides a foundational dataset for investigating the mechanisms of phytoplasma transmission, comparative genomics within the Deltocephalinae subfamily, and the development of targeted pest management strategies.