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<p>RNA modifications represent a crucial layer of post-transcriptional regulation, yet their dynamic landscape during testicular maturation remains largely uncharted. In this study, we employed Nanopore Direct RNA Sequencing to generate a comprehensive epitranscriptomic dataset for porcine testicular tissue at pre-sexual and post-sexual maturation stages. This dataset captures the profiles of four RNA modifications (m⁶A, m⁵C, Ψ, and inosine) across these critical developmental time points. We further characterized the global abundance, distribution, and motif features of these modifications. This resource provides a valuable foundation for elucidating the epitranscriptomic mechanisms governing mammalian testicular development and spermatogenesis.</p>

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A Nanopore Direct RNA Sequencing Atlas of RNA Modifications in pig (Sus scrofa domesticus) Testes across Sexual Maturation

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年6月29日

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  • Yiting Yang,
  • Zhijuan Yan,
  • Ziling Hao,
  • Junxiang Mao,
  • Yong Du,
  • Yan Wang,
  • Xiaofeng Zhou,
  • Lei Chen,
  • Ye Zhao,
  • Linyuan Shen,
  • Li Zhu,
  • Mailin Gan

摘要

RNA modifications represent a crucial layer of post-transcriptional regulation, yet their dynamic landscape during testicular maturation remains largely uncharted. In this study, we employed Nanopore Direct RNA Sequencing to generate a comprehensive epitranscriptomic dataset for porcine testicular tissue at pre-sexual and post-sexual maturation stages. This dataset captures the profiles of four RNA modifications (m⁶A, m⁵C, Ψ, and inosine) across these critical developmental time points. We further characterized the global abundance, distribution, and motif features of these modifications. This resource provides a valuable foundation for elucidating the epitranscriptomic mechanisms governing mammalian testicular development and spermatogenesis.