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<p><i>Serrodes campana</i> (Lepidoptera: Noctuidae) is an economically important moth distributed in subtropical and tropical Asia, but genomic resources for this species have been lacking. Here, we present a chromosome-level reference genome assembly for <i>S. campana</i> generated using PacBio HiFi long reads, Illumina short reads, Hi-C sequencing, and transcriptomic data from 12 RNA-seq samples. The final assembly spans 627.9 Mb, with a contig N50 of 21.4 Mb and a scaffold N50 of 21.6 Mb, and 99.29% of the assembled sequences were anchored to 31 pseudochromosomes. BUSCO assessment recovered 99.56% complete insect genes, supporting the high completeness of the assembly. We predicted 16,047 protein-coding genes, of which 96.65% were functionally annotated, and identified repetitive elements accounting for 33.79% of the genome. This genome assembly and associated annotation resources provide a foundation for gene annotation, comparative genomics, genome evolution studies, and investigations of host-associated biology in <i>S. campana</i> and related moth species.</p>

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A chromosome-level reference genome assembly and annotation of Serrodes campana

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年6月17日

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  • Lu Li,
  • Junbao Wen,
  • Liming Jia

摘要

Serrodes campana (Lepidoptera: Noctuidae) is an economically important moth distributed in subtropical and tropical Asia, but genomic resources for this species have been lacking. Here, we present a chromosome-level reference genome assembly for S. campana generated using PacBio HiFi long reads, Illumina short reads, Hi-C sequencing, and transcriptomic data from 12 RNA-seq samples. The final assembly spans 627.9 Mb, with a contig N50 of 21.4 Mb and a scaffold N50 of 21.6 Mb, and 99.29% of the assembled sequences were anchored to 31 pseudochromosomes. BUSCO assessment recovered 99.56% complete insect genes, supporting the high completeness of the assembly. We predicted 16,047 protein-coding genes, of which 96.65% were functionally annotated, and identified repetitive elements accounting for 33.79% of the genome. This genome assembly and associated annotation resources provide a foundation for gene annotation, comparative genomics, genome evolution studies, and investigations of host-associated biology in S. campana and related moth species.