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<p><i>Corydalis saxicola</i>, an endangered herbaceous plant belonging to the Papaveraceae family and used traditionally as folk medicine, is exclusively endemic to karst habitats. However, the lack of a reference genome limits the implementation of molecular techniques in its breeding, pharmacology and domestication. Here, we present a high-quality chromosome-level genome assembly of <i>C. saxicola</i> based on PacBio HiFi and Hi-C data. The assembled genome size is 240.94 Mb with a contig N50 of 29.21 Mb and BUSCO completeness of 97.71%. Approximately 93.26% of the assembled sequences could be anchored to eight pseudo-chromosomes. A total of 74.29 Mb repeat sequences were identified, which account for 32.33% of the genome. In addition, 24,203 protein-coding genes were identified with a BUSCO completeness of 97.89%. This high-quality genome assembly will serve as a valuable resource for understanding the ecology, genetics, and evolution of <i>C. Saxicola</i> and will help towards its cultivation.</p>

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Chromosome-level genome assembly and annotation of the endemic and endangered karst medicinal plant Corydalis saxicola

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年8月5日

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  • Ming Lei,
  • Jing Wang,
  • Suren Sooranna,
  • Han Liu,
  • Zhao-di Wen,
  • Liang Kang,
  • Mei Qin,
  • Xia-lian Ou,
  • Li-rong Huang,
  • Ying Wei,
  • Cui Li,
  • Zhan-jiang Zhang

摘要

Corydalis saxicola, an endangered herbaceous plant belonging to the Papaveraceae family and used traditionally as folk medicine, is exclusively endemic to karst habitats. However, the lack of a reference genome limits the implementation of molecular techniques in its breeding, pharmacology and domestication. Here, we present a high-quality chromosome-level genome assembly of C. saxicola based on PacBio HiFi and Hi-C data. The assembled genome size is 240.94 Mb with a contig N50 of 29.21 Mb and BUSCO completeness of 97.71%. Approximately 93.26% of the assembled sequences could be anchored to eight pseudo-chromosomes. A total of 74.29 Mb repeat sequences were identified, which account for 32.33% of the genome. In addition, 24,203 protein-coding genes were identified with a BUSCO completeness of 97.89%. This high-quality genome assembly will serve as a valuable resource for understanding the ecology, genetics, and evolution of C. Saxicola and will help towards its cultivation.