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<p><i>Rosa anemoniflora</i>, a critically endangered climbing rose endemic to China, holds significant ornamental and conservation value but lacks genomic resources. Here, we present a chromosome-level genome assembly generated using PacBio HiFi, Illumina, Iso-Seq, and Hi-C sequencing. The final assembly spans 547.52 Mb, with 95.72% of sequences anchored to seven pseudochromosomes and a contig N50 of 73.49 Mb. BUSCO assessment reveals 97.36% completeness (eudicotyledons_odb12). Repetitive elements constitute 55.31% of the genome, and 33,623 protein-coding genes were annotated, 97.68% of which are supported by BUSCO. We also identified 1,333 rRNA, 838 tRNA, and 1,043 other non-coding RNA genes. All data are publicly available at National Genomics Data Center (NGDC) (PRJCA052329). This high-quality genome provides a foundational resource for conservation genomics, evolutionary studies, and the genetic dissection of adaptive and reproductive traits in this threatened species.</p>

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A chromosome-level genome assembly of the endangered rose Rosa anemoniflora

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年6月1日

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  • Xianzhen Zhou,
  • Qun Yu,
  • Chengjie Fu,
  • Guojiang Lin,
  • Xiaochun Huang,
  • Tianqi Kang,
  • Wei Gao

摘要

Rosa anemoniflora, a critically endangered climbing rose endemic to China, holds significant ornamental and conservation value but lacks genomic resources. Here, we present a chromosome-level genome assembly generated using PacBio HiFi, Illumina, Iso-Seq, and Hi-C sequencing. The final assembly spans 547.52 Mb, with 95.72% of sequences anchored to seven pseudochromosomes and a contig N50 of 73.49 Mb. BUSCO assessment reveals 97.36% completeness (eudicotyledons_odb12). Repetitive elements constitute 55.31% of the genome, and 33,623 protein-coding genes were annotated, 97.68% of which are supported by BUSCO. We also identified 1,333 rRNA, 838 tRNA, and 1,043 other non-coding RNA genes. All data are publicly available at National Genomics Data Center (NGDC) (PRJCA052329). This high-quality genome provides a foundational resource for conservation genomics, evolutionary studies, and the genetic dissection of adaptive and reproductive traits in this threatened species.