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<p>Non-coding RNAs play essential roles during liver regeneration, however, their functions in cholangiocyte-to-hepatocyte differentiation is still unclear. Emerging liver ductal organoids offer a powerful model to replicate the molecular mechanisms of the <i>in vivo</i> cholangiocyte-to-immature hepatocyte transition, potentially accelerating advancements in liver regenerative medicine. Here, we established the liver ductal organoids from three C57BL/6 mice, and induced the differentiation of cholangiocyte into immature hepatocyte to carry out the transcriptome sequencing. Then, the expression profiles of lncRNA, miRNA and mRNA in expansion (EM) and differentiation medium (DM) samples were explored. A total of 529,049,578 and 61,266,130 clean reads were generated from 6 RNA-seq and 6 miRNA-seq libraries, respectively. Through stringent filtering, we identified 24,676 lncRNAs, 1,018 miRNAs and 38,689 mRNAs transcripts. This dataset will contribute to the elucidation of the underlying molecular mechanisms associated with cholangiocyte-to-immature hepatocyte differentiation during liver regeneration, and provides clues for liver regenerative medicine.</p>

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lncRNA, miRNA, and mRNA dynamics in cholangiocyte-to-immature hepatocyte differentiation in liver regeneration

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年5月16日

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  • Pingping Huang,
  • Xue Yu,
  • Congcong Shen,
  • Zanxia Cao,
  • Bailing Zhou,
  • Ruiping Wang,
  • Jiarong Wang,
  • Jihua Wang

摘要

Non-coding RNAs play essential roles during liver regeneration, however, their functions in cholangiocyte-to-hepatocyte differentiation is still unclear. Emerging liver ductal organoids offer a powerful model to replicate the molecular mechanisms of the in vivo cholangiocyte-to-immature hepatocyte transition, potentially accelerating advancements in liver regenerative medicine. Here, we established the liver ductal organoids from three C57BL/6 mice, and induced the differentiation of cholangiocyte into immature hepatocyte to carry out the transcriptome sequencing. Then, the expression profiles of lncRNA, miRNA and mRNA in expansion (EM) and differentiation medium (DM) samples were explored. A total of 529,049,578 and 61,266,130 clean reads were generated from 6 RNA-seq and 6 miRNA-seq libraries, respectively. Through stringent filtering, we identified 24,676 lncRNAs, 1,018 miRNAs and 38,689 mRNAs transcripts. This dataset will contribute to the elucidation of the underlying molecular mechanisms associated with cholangiocyte-to-immature hepatocyte differentiation during liver regeneration, and provides clues for liver regenerative medicine.