Scholarlink

<p>The Tibetan wild donkey (<i>Equus kiang</i>) is an endemic equid species inhabiting the Qinghai–Tibet Plateau, exhibiting remarkable chromosomal diversity within the <i>Equus</i> genus. Here we present a chromosome-level, telomere-to-telomere genome assembly for <i>E. kiang</i> generated using Illumina short reads, PacBio HiFi long reads, and Hi-C sequencing data. The final assembly spans 4.02 Gb and comprises 27 pseudochromosomes. Specifically, 8 chromosomes are gapless, 8 chromosomes have one gap, 7 chromosomes have 2–4 gaps, and the remaining 4 chromosomes have five or more gaps. Benchmarking Universal Single-Copy Orthologs (BUSCO) analysis indicated 96.7% completeness, demonstrating high assembly quality. Comparative genome collinearity with the domestic horse (E. caballus) showed that 11 <i>E. kiang</i> chromosomes displayed a simple one-to-one chromosome-scale correspondence. We further characterized genomic features associated with these regions, including the enrichment of tandem repeats near recombination breakpoints. This high-quality genome assembly provides a foundational resource for studies of chromosome evolution, speciation, and genomic architecture in <i>Equus</i> and other mammals.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Telomere-to-telomere Genome Assembly of Equus kiang (Tibetan wild donkey)

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年5月6日

查看全文
  • Yanling Zheng,
  • Yue Hu,
  • Jiajia Suo,
  • Wenchao Wang,
  • Yunzhou Wang,
  • Lei Cao,
  • Peng Sun,
  • Maishou Zhang,
  • Manda Zhao,
  • Wanjiao Yang,
  • Xiu Li,
  • Bayi Jiang,
  • Xun Bu,
  • Xiaoxia Du,
  • Quanfang Zhang

摘要

The Tibetan wild donkey (Equus kiang) is an endemic equid species inhabiting the Qinghai–Tibet Plateau, exhibiting remarkable chromosomal diversity within the Equus genus. Here we present a chromosome-level, telomere-to-telomere genome assembly for E. kiang generated using Illumina short reads, PacBio HiFi long reads, and Hi-C sequencing data. The final assembly spans 4.02 Gb and comprises 27 pseudochromosomes. Specifically, 8 chromosomes are gapless, 8 chromosomes have one gap, 7 chromosomes have 2–4 gaps, and the remaining 4 chromosomes have five or more gaps. Benchmarking Universal Single-Copy Orthologs (BUSCO) analysis indicated 96.7% completeness, demonstrating high assembly quality. Comparative genome collinearity with the domestic horse (E. caballus) showed that 11 E. kiang chromosomes displayed a simple one-to-one chromosome-scale correspondence. We further characterized genomic features associated with these regions, including the enrichment of tandem repeats near recombination breakpoints. This high-quality genome assembly provides a foundational resource for studies of chromosome evolution, speciation, and genomic architecture in Equus and other mammals.