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<p>Liver progenitor-like cells (LPLCs) are essential for liver regeneration during some injury process, yet their epigenetic characters remain poorly understood. Using single-nucleus assay for transposase-accessible chromatin sequencing (snATAC-seq), we profiled chromatin accessibility in a mouse model of 3,5-diethoxycarbonyl-1,4-dihydrocollidin (DDC) diet-induced cholestasis at six time points: homeostasis (D0), injury (D8, D17), and repair (R2, R7, R21). We analyzed 75,452 high-quality nuclei, identifying 15 liver cell types, including LPLCs. Among 221,845 accessible chromatin regions, 192,079 showed differential accessibility. LPLCs exhibited enriched binding motifs for SWI/SNF (SMARCC1) and AP-1 (FOS, JUND and JUNB) complexes, highlighting their roles in transcriptional regulation. This study provides a comprehensive chromatin accessibility landscape of liver injury and regeneration, suggesting SWI/SNF and AP-1 as potential therapeutic targets.</p>

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Single-nucleus chromatin landscapes of cholestatic injury and repair in mice liver

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年5月2日

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  • Hong Wu,
  • Yongqing Yang,
  • Yan Wu,
  • Jiangshan Xu,
  • Pengcheng Guo,
  • Shiping Liu,
  • Yong Hou,
  • Shijie Hao

摘要

Liver progenitor-like cells (LPLCs) are essential for liver regeneration during some injury process, yet their epigenetic characters remain poorly understood. Using single-nucleus assay for transposase-accessible chromatin sequencing (snATAC-seq), we profiled chromatin accessibility in a mouse model of 3,5-diethoxycarbonyl-1,4-dihydrocollidin (DDC) diet-induced cholestasis at six time points: homeostasis (D0), injury (D8, D17), and repair (R2, R7, R21). We analyzed 75,452 high-quality nuclei, identifying 15 liver cell types, including LPLCs. Among 221,845 accessible chromatin regions, 192,079 showed differential accessibility. LPLCs exhibited enriched binding motifs for SWI/SNF (SMARCC1) and AP-1 (FOS, JUND and JUNB) complexes, highlighting their roles in transcriptional regulation. This study provides a comprehensive chromatin accessibility landscape of liver injury and regeneration, suggesting SWI/SNF and AP-1 as potential therapeutic targets.