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<p><i>Halisarca dujardinii</i> is a marine sponge known for its ability to completely regenerate via cell reaggregation. Here we present the first chromosome-level genome assembly of <i>H. dujardinii</i>, generated using Oxford Nanopore long reads, Illumina short reads, and Hi-C data. The final assembly spans 226.5 Mbp and is organized into 21 chromosome-scale scaffolds, representing the full nuclear genome, with an assembly N50 of 10.1 Mbp. In addition, we report the complete mitochondrial genome for <i>H. dujardinii</i>, assembled as a circular molecule and annotated to contain 14 protein-coding genes. We provide a comprehensive genome annotation comprising 14,565 nuclear protein-coding genes, of which 85.6% are functionally annotated, along with repetitive elements and non-coding RNAs. Transcript models were refined using extensive bulk and single-cell RNA sequencing data, enabling accurate annotation of 3′ untranslated regions. This new genomic resource provides a valuable foundation for investigating the molecular basis of sponge regeneration, as well as for comparative studies of sponge evolution and marine biology.</p>

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Chromosome-level genome assembly of the sponge Halisarca dujardinii

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年4月21日

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  • Vasiliy Zubarev,
  • Alexander Cherkasov,
  • Leonid Sidorov,
  • Kim I. Adameyko,
  • Alexander D. Finoshin,
  • Alina Ryabova,
  • Anastasiia Kashtanova,
  • Guzel Gazizova,
  • Natalia Gogoleva,
  • Anton V. Burakov,
  • Olga Kozlova,
  • Elena Shagimardanova,
  • Oleg Gusev,
  • Kirill Mikhailov,
  • Oksana Kravchuk,
  • Yulia Lyupina,
  • Ekaterina Khrameeva

摘要

Halisarca dujardinii is a marine sponge known for its ability to completely regenerate via cell reaggregation. Here we present the first chromosome-level genome assembly of H. dujardinii, generated using Oxford Nanopore long reads, Illumina short reads, and Hi-C data. The final assembly spans 226.5 Mbp and is organized into 21 chromosome-scale scaffolds, representing the full nuclear genome, with an assembly N50 of 10.1 Mbp. In addition, we report the complete mitochondrial genome for H. dujardinii, assembled as a circular molecule and annotated to contain 14 protein-coding genes. We provide a comprehensive genome annotation comprising 14,565 nuclear protein-coding genes, of which 85.6% are functionally annotated, along with repetitive elements and non-coding RNAs. Transcript models were refined using extensive bulk and single-cell RNA sequencing data, enabling accurate annotation of 3′ untranslated regions. This new genomic resource provides a valuable foundation for investigating the molecular basis of sponge regeneration, as well as for comparative studies of sponge evolution and marine biology.