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<p>The Leschenault’s rousette (<i>Rousettus leschenaultii</i>) represents a medium-sized bat of the genus <i>Rousettus</i> that feeds on fruits or flowers, mainly distributed in Southeast Asia. Here, we generated a chromosome-level genome assembly of <i>R. leschenaultii</i> using a hybrid MGI and PacBio sequencing approach, facilitated by the chromosome assignment using the high-throughput chromatin conformation capture (Hi-C) sequencing technology. The genome size was 1.95 Gb with a scaffold N50 of 116.99 Mb, and 99.00% of the assembled sequences were anchored to 17 autosomes and the two sex chromosomes (X and Y). The completeness of the assembly was estimated to be 96.4% using BUSCO. In total, 19,625 genes were predicted from this genome assembly, with 97.83% of them being functionally annotated. This high-quality assembly of <i>R. leschenaultii</i> serves as a valuable genetic resource for exploring the genomic basis of evolutionary adaptation, and for conducting population, ecological, and conservation genomic studies.</p>

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A chromosome-level genome assembly of the Leschenault’s rousette (Rousettus leschenaultii)

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年3月13日

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  • Lijia Chen,
  • Guang Yang,
  • Shengwei Hou,
  • Simin Chai

摘要

The Leschenault’s rousette (Rousettus leschenaultii) represents a medium-sized bat of the genus Rousettus that feeds on fruits or flowers, mainly distributed in Southeast Asia. Here, we generated a chromosome-level genome assembly of R. leschenaultii using a hybrid MGI and PacBio sequencing approach, facilitated by the chromosome assignment using the high-throughput chromatin conformation capture (Hi-C) sequencing technology. The genome size was 1.95 Gb with a scaffold N50 of 116.99 Mb, and 99.00% of the assembled sequences were anchored to 17 autosomes and the two sex chromosomes (X and Y). The completeness of the assembly was estimated to be 96.4% using BUSCO. In total, 19,625 genes were predicted from this genome assembly, with 97.83% of them being functionally annotated. This high-quality assembly of R. leschenaultii serves as a valuable genetic resource for exploring the genomic basis of evolutionary adaptation, and for conducting population, ecological, and conservation genomic studies.