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<p>The pecan weevil, <i>Curculio caryae</i> (Horn), is an obligate feeder of pecan and native hickory trees (genus <i>Carya</i>) throughout North America. Subsequently it is a significant agricultural pest in pecan orchards. In this study, we present a reference quality genome using deep-coverage, ~40x PacBio HiFi genome sequence reads, and chromatin confirmation, Hi-C, scaffolding. The final genome assembly is approximately 2.2 Gb, which was confirmed by flow cytometry. The primary genome scaffolds have an N50 of 132 Mb and a BUSCO completeness of 95.4% [S:94.3%, D:1.1%]. Furthermore, we employed PacBio long-read RNA, Iso-seq, for <i>de novo</i> gene annotation, in conjunction with InterProscan to identify approximately 19,000 protein coding genes. Repeat content is extensive, contributing at least &gt;80% of the total genome. This data set provides a valuable resource for comparative genomics and evolutionary studies of an economically impactful group of insect pests that currently lack extensive genomic resources.</p>

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A chromosome level reference genome for the pecan weevil, Curculio caryae

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年3月18日

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  • Lindsey C. Perkin,
  • Zachary P. Cohen,
  • Sheina B. Sim,
  • Scott M. Geib,
  • Anna K. Childers,
  • Timothy P. L. Smith,
  • J. Spencer Johnston,
  • Perot Saelao,
  • Charles P.-C. Suh

摘要

The pecan weevil, Curculio caryae (Horn), is an obligate feeder of pecan and native hickory trees (genus Carya) throughout North America. Subsequently it is a significant agricultural pest in pecan orchards. In this study, we present a reference quality genome using deep-coverage, ~40x PacBio HiFi genome sequence reads, and chromatin confirmation, Hi-C, scaffolding. The final genome assembly is approximately 2.2 Gb, which was confirmed by flow cytometry. The primary genome scaffolds have an N50 of 132 Mb and a BUSCO completeness of 95.4% [S:94.3%, D:1.1%]. Furthermore, we employed PacBio long-read RNA, Iso-seq, for de novo gene annotation, in conjunction with InterProscan to identify approximately 19,000 protein coding genes. Repeat content is extensive, contributing at least >80% of the total genome. This data set provides a valuable resource for comparative genomics and evolutionary studies of an economically impactful group of insect pests that currently lack extensive genomic resources.