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<p>Siberian kale (<i>Brassica napus</i> subsp. <i>pabularia</i>, AACC, 2n = 38) is a distinct subspecies of <i>B. napus</i>, characterized by its deeply lobed leaves and primarily cultivated as a nutritious leafy vegetable. Here, we present a chromosome-level genome of Beta, a Siberian kale variety, integrating Illumina short reads, PacBio HiFi long reads, and Hi-C data. The final assembly size is 1,078.8 Mb, with a scaffold N50 of 57.5 Mb and a genome BUSCO completeness of 99.7%. 954.0 Mb (88.4%) of sequences were successfully anchored to 19 pseudo-chromosomes. The configuration of Beta genome chromosomes is consistent with the distribution of ten A subgenome and nine C subgenome chromosomes in rapeseed. In total, 98,882 protein-coding genes were predicted <i>ab initio</i> in the Beta genome, with an average gene length of 1,997 bp, and 90,415 (91.44%) genes were functionally annotated. Overall, the high-quality genome provides a valuable resource for bridging current knowledge gaps and offers key genetic insights into deeply lobed leaf formation and improvement of <i>Brassica</i> crops.</p>

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Chromosome-level genome assembly of Siberian kale (Brassica napus subsp. pabularia)

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年2月27日

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  • Xi Shan,
  • Minghao Qu,
  • Wei Zhang,
  • Shenyun Wang,
  • Fangwei Yu,
  • Meng Ni,
  • Lei Gao,
  • Xiaolin Yu,
  • Jianbin Li

摘要

Siberian kale (Brassica napus subsp. pabularia, AACC, 2n = 38) is a distinct subspecies of B. napus, characterized by its deeply lobed leaves and primarily cultivated as a nutritious leafy vegetable. Here, we present a chromosome-level genome of Beta, a Siberian kale variety, integrating Illumina short reads, PacBio HiFi long reads, and Hi-C data. The final assembly size is 1,078.8 Mb, with a scaffold N50 of 57.5 Mb and a genome BUSCO completeness of 99.7%. 954.0 Mb (88.4%) of sequences were successfully anchored to 19 pseudo-chromosomes. The configuration of Beta genome chromosomes is consistent with the distribution of ten A subgenome and nine C subgenome chromosomes in rapeseed. In total, 98,882 protein-coding genes were predicted ab initio in the Beta genome, with an average gene length of 1,997 bp, and 90,415 (91.44%) genes were functionally annotated. Overall, the high-quality genome provides a valuable resource for bridging current knowledge gaps and offers key genetic insights into deeply lobed leaf formation and improvement of Brassica crops.