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<p>The silver fir, <i>Abies alba</i> Mill., is a key species in European mountain forest ecosystems, which, despite being one of the few conifers with an available reference genome, lacked high quality transcriptomic information. Here, we present a <i>de novo</i> transcriptome assembly of this species, obtained using both short- and long-read sequencing data, from three different organs: leaves, stem, and roots. Saplings were subjected to different treatments, including cold, heat, drought, and darkness, in order to induce the expression of as many genes as possible to obtain a highly comprehensive representation of the silver fir’s transcriptome. The assembled transcriptome shows a completeness value of 94.8%, with 539,313 assembled transcripts, of which approximately 30% were functionally annotated. It constitutes the highest quality transcriptome available to date for <i>A. alba</i>, providing a valuable tool for further studies involving this species.</p>

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De novo transcriptome assembly of the silver fir, Abies alba Mill

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年2月7日

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  • Isabel García-García,
  • Belén Méndez-Cea,
  • Jose Luis Horreo,
  • José Ignacio Seco,
  • Juan Carlos Linares,
  • Francisco Javier Gallego

摘要

The silver fir, Abies alba Mill., is a key species in European mountain forest ecosystems, which, despite being one of the few conifers with an available reference genome, lacked high quality transcriptomic information. Here, we present a de novo transcriptome assembly of this species, obtained using both short- and long-read sequencing data, from three different organs: leaves, stem, and roots. Saplings were subjected to different treatments, including cold, heat, drought, and darkness, in order to induce the expression of as many genes as possible to obtain a highly comprehensive representation of the silver fir’s transcriptome. The assembled transcriptome shows a completeness value of 94.8%, with 539,313 assembled transcripts, of which approximately 30% were functionally annotated. It constitutes the highest quality transcriptome available to date for A. alba, providing a valuable tool for further studies involving this species.