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<p>Cold stimulation has emerged as a promising therapeutic strategy against obesity by activating thermogenesis. Although the metabolic responses of adipose tissue to cold are well documented, the dynamic transcriptional and proteomic changes that occur in both inguinal white adipose tissue (iWAT) and classic brown adipose tissue (BAT) in response to cold exposure remain poorly understood. To elucidate the mechanisms underlying cold-induced thermogenesis and adipose tissue remodeling, we used RNA sequencing (RNA-seq) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) to examine the transcriptomic and proteomic profiles, respectively, of adipose tissue from mice exposed to cold or room temperature. The male C57BL/6 J mice were divided into three groups (5 mice/group), two groups were kept at 6 °C for 6 h and 24 h, respectively, and the control group was kept at 22 °C. Subsequently, the BAT and iWAT of each mouse were dissected and subjected to RNA-seq and data-independent acquisition (DIA)-based LC-MS/MS. All data are publicly available and can be used to identify key genes and signaling pathways affected by cold exposure.</p>

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Temporal transcriptomic and proteomic characterization of adipose tissue from cold-exposed mice

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年2月2日

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  • Qiuyan Zhu,
  • Shuo Wang,
  • Hao Zhou,
  • Jianbo Yang,
  • Yangya Liang,
  • Xi Chen,
  • Jianlin Zhou,
  • Dandan Wu,
  • Xing Zhang

摘要

Cold stimulation has emerged as a promising therapeutic strategy against obesity by activating thermogenesis. Although the metabolic responses of adipose tissue to cold are well documented, the dynamic transcriptional and proteomic changes that occur in both inguinal white adipose tissue (iWAT) and classic brown adipose tissue (BAT) in response to cold exposure remain poorly understood. To elucidate the mechanisms underlying cold-induced thermogenesis and adipose tissue remodeling, we used RNA sequencing (RNA-seq) and liquid chromatography-tandem mass spectrometry (LC-MS/MS) to examine the transcriptomic and proteomic profiles, respectively, of adipose tissue from mice exposed to cold or room temperature. The male C57BL/6 J mice were divided into three groups (5 mice/group), two groups were kept at 6 °C for 6 h and 24 h, respectively, and the control group was kept at 22 °C. Subsequently, the BAT and iWAT of each mouse were dissected and subjected to RNA-seq and data-independent acquisition (DIA)-based LC-MS/MS. All data are publicly available and can be used to identify key genes and signaling pathways affected by cold exposure.