Scholarlink

<p>As a First-Class Protected species in China, the Tibetan wild ass (<i>Equus kiang</i>) is endemic to the Qinghai-Tibet Plateau and exhibits remarkable adaptation to extreme high-altitude environments characterized by low oxygen, cold temperatures, and high UV radiation. To explore its genetic basis and evolutionary adaptations, we generated the first telomere-to-telomere (T2T) gapless genome using an integrated sequencing strategy that combined PacBio HiFi, ultra-long Oxford Nanopore (ONT), Hi-C, and BGI short-read technologies. The resulting 2.49 Gb assembly achieved a contig N50 of 107.02 Mb, comprising all 54 telomeres and 27 centromeres across 25 pairs of autosomes and the X/Y chromosomes, and reached 99.94% BUSCO completeness. Genome annotation predicted 907 Mb of repetitive sequences (36.52% of the genome) and 24,005 protein-coding genes. Comparative and technical analyses confirmed high assembly continuity, completeness, and accuracy, with a consensus quality value (QV) of 64.73, providing a robust genomic reference for understanding the evolutionary mechanisms and adaptive traits of this iconic high-altitude ungulate.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

A telomere-to-telomere gapless genome assembly of the Tibetan wild ass (Equus kiang)

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2026年1月6日

查看全文
  • Bilige Wuyun,
  • Jie Liu,
  • Rihan Wu,
  • Yunxia Li,
  • Fangyuan Liu,
  • Hengquan Zhao,
  • Chunxia Hao,
  • Gaoping Zhao,
  • Wei Sun,
  • Yongli Song,
  • Wei Wang,
  • Yu Wang,
  • Cunxin Ma,
  • Fengyi Xu,
  • Jian He,
  • Pengyue Wang,
  • Xiangnan Bao,
  • Guifang Cao,
  • Yong Zhang,
  • Ying Lu,
  • Xihe Li

摘要

As a First-Class Protected species in China, the Tibetan wild ass (Equus kiang) is endemic to the Qinghai-Tibet Plateau and exhibits remarkable adaptation to extreme high-altitude environments characterized by low oxygen, cold temperatures, and high UV radiation. To explore its genetic basis and evolutionary adaptations, we generated the first telomere-to-telomere (T2T) gapless genome using an integrated sequencing strategy that combined PacBio HiFi, ultra-long Oxford Nanopore (ONT), Hi-C, and BGI short-read technologies. The resulting 2.49 Gb assembly achieved a contig N50 of 107.02 Mb, comprising all 54 telomeres and 27 centromeres across 25 pairs of autosomes and the X/Y chromosomes, and reached 99.94% BUSCO completeness. Genome annotation predicted 907 Mb of repetitive sequences (36.52% of the genome) and 24,005 protein-coding genes. Comparative and technical analyses confirmed high assembly continuity, completeness, and accuracy, with a consensus quality value (QV) of 64.73, providing a robust genomic reference for understanding the evolutionary mechanisms and adaptive traits of this iconic high-altitude ungulate.