Scholarlink

<p>Oyster pompano (<i>Trachinotus anak</i>) is currently the highest-yielding marine aquaculture fish species in China. However, previously available genome assemblies were fragmented and incomplete, hindering advances in genomic research and breeding applications. Here, we generated two haplotype-resolved, telomere-to-telomere (T2T) genome assemblies for a female <i>T. anak</i> using PacBio HiFi, ONT ultra-long, and Hi-C data. The resulting assemblies (haplotype A and B) span 663.78 Mb and 661.09 Mb with contig N50 of 28.62 Mb and 29.02 Mb, respectively. Both haplotypic assemblies were anchored into 24 gap-free chromosomes, each comprising a complete set of 48 telomeres. The quality value (QV) scores were 70.45 and 68.66, and Benchmarking Universal Single-Copy Orthologs (BUSCO) completeness scores were 98.9% and 99.0% for haplotype A and B, respectively. Genome annotation identified 187.94 Mb and 178.63 Mb of repetitive sequences, and predicted 23,118 and 23,119 protein-coding genes in haplotypes A and B, respectively, with 99.79% and 99.78% functionally annotated. These two high-quality T2T assemblies provide invaluable resources for molecular breeding and advancing biological and evolutionary research in <i>T. anak</i>.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Telomere-to-telomere haplotype-resolved genome assembly of a female oyster pompano (Trachinotus anak)

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年12月2日

查看全文
  • Tao Wang,
  • Yuwen Guo,
  • Yan Wang,
  • Rong Chen,
  • Si Ge,
  • Huapu Chen

摘要

Oyster pompano (Trachinotus anak) is currently the highest-yielding marine aquaculture fish species in China. However, previously available genome assemblies were fragmented and incomplete, hindering advances in genomic research and breeding applications. Here, we generated two haplotype-resolved, telomere-to-telomere (T2T) genome assemblies for a female T. anak using PacBio HiFi, ONT ultra-long, and Hi-C data. The resulting assemblies (haplotype A and B) span 663.78 Mb and 661.09 Mb with contig N50 of 28.62 Mb and 29.02 Mb, respectively. Both haplotypic assemblies were anchored into 24 gap-free chromosomes, each comprising a complete set of 48 telomeres. The quality value (QV) scores were 70.45 and 68.66, and Benchmarking Universal Single-Copy Orthologs (BUSCO) completeness scores were 98.9% and 99.0% for haplotype A and B, respectively. Genome annotation identified 187.94 Mb and 178.63 Mb of repetitive sequences, and predicted 23,118 and 23,119 protein-coding genes in haplotypes A and B, respectively, with 99.79% and 99.78% functionally annotated. These two high-quality T2T assemblies provide invaluable resources for molecular breeding and advancing biological and evolutionary research in T. anak.