<p>Amur minnow (<i>Rhynchocypris lagowskii</i>), an economically important freshwater fish, inhabits tributaries of the Heilongjiang River to Yangtze River in China. Thus far, no genome data of this species have been reported. In this study, we constructed chromosome-level genome assembly for <i>R. lagowskii</i> by integration of MGI, PacBio HiFi and Hi-C sequencing technologies. The assembled genome was 1,041.22 Mb in size, with the scaffold N50 of 32.63 Mb, and 99.04% of the sequences (1,007.75 Mb) were anchored to 25 chromosomes. A total of 32,425 protein-coding genes were predicted in the final assembly, of which 91.09% were functionally annotated. The BUSCO score of genome assembly and protein annotation were 96.32% and 97.53%, respectively, indicating a high quality of genome assembly. The high-quality genome serves as a critical resource for in-depth investigations on comparative genomics, population genetics, molecular breeding, and functional exploration of this economically important fish.</p>

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Chromosome-level genome assembly of Amur Minnow (Rhynchocypris lagowskii)

  • Xiaohui Bai,
  • Hanlin Li,
  • Yaoxin Wang,
  • Xinhui Zhang,
  • Jingjing Li,
  • Xin Luo,
  • Zhang Luo,
  • Lin Ma,
  • Shuang Hao,
  • Chen Wu,
  • Wei Zhang

摘要

Amur minnow (Rhynchocypris lagowskii), an economically important freshwater fish, inhabits tributaries of the Heilongjiang River to Yangtze River in China. Thus far, no genome data of this species have been reported. In this study, we constructed chromosome-level genome assembly for R. lagowskii by integration of MGI, PacBio HiFi and Hi-C sequencing technologies. The assembled genome was 1,041.22 Mb in size, with the scaffold N50 of 32.63 Mb, and 99.04% of the sequences (1,007.75 Mb) were anchored to 25 chromosomes. A total of 32,425 protein-coding genes were predicted in the final assembly, of which 91.09% were functionally annotated. The BUSCO score of genome assembly and protein annotation were 96.32% and 97.53%, respectively, indicating a high quality of genome assembly. The high-quality genome serves as a critical resource for in-depth investigations on comparative genomics, population genetics, molecular breeding, and functional exploration of this economically important fish.