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<p>The functional complexity of the mammalian brain is rooted in the diverse protein composition of synapses across distinct regions. To address the need for a detailed molecular understanding of this regional diversity, we generated the first comprehensive proteomic map of synaptosomes from six key rat brain regions: anterior cortex, posterior cortex, hippocampus, striatum, olfactory bulb, and cerebellum. Using high-resolution quantitative proteome and the Percoll density gradient ultracentrifugation, we identified 3,440 proteins and yielded high synaptic enrichment (60.11% synapse-unique proteins). In-depth functional analysis using the SynGO database revealed region-specific enrichments in the cellular components and biological processes. This study provides a valuable resource and foundational insights into the molecular architecture underlying regional synaptic diversity, paving the way for future investigations into brain function and neurological disorders.</p>

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A Resource for Exploring Regional Diversity in the Male Rat Brain Synaptic Proteome

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年8月27日

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  • Yuran Wang,
  • Yunshan Li,
  • Xiaolin Tian,
  • Yiying Wu,
  • Yahui Li,
  • Xuewei Zhao,
  • Yuhang Cai,
  • Panpan Zhang,
  • Wei Zhang,
  • Dezhi Kong

摘要

The functional complexity of the mammalian brain is rooted in the diverse protein composition of synapses across distinct regions. To address the need for a detailed molecular understanding of this regional diversity, we generated the first comprehensive proteomic map of synaptosomes from six key rat brain regions: anterior cortex, posterior cortex, hippocampus, striatum, olfactory bulb, and cerebellum. Using high-resolution quantitative proteome and the Percoll density gradient ultracentrifugation, we identified 3,440 proteins and yielded high synaptic enrichment (60.11% synapse-unique proteins). In-depth functional analysis using the SynGO database revealed region-specific enrichments in the cellular components and biological processes. This study provides a valuable resource and foundational insights into the molecular architecture underlying regional synaptic diversity, paving the way for future investigations into brain function and neurological disorders.