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<p>The leaf wax layer in cauliflower (<i>Brassica oleracea var. Botrytis</i> L.) and other <i>Brassica</i> crops play an important role in environmental adaptation and defense. Here, high-throughput RNA sequencing was performed on leaves of a wax-deficient mutant type (WL) and its wild type (YL). A total of 43.13 Gb of raw RNA-seq data was obtained, of which 42.38 Gb of high-quality clean reads were retained after quality control. A total of 24,529 genes and 1,092 long non-coding RNA (lncRNA) were identified through&#xa0;transcriptome assembly and annotation. Functional enrichment analysis indicated that these mRNA and lncRNA are associated with biotic stress responses, lipid biosynthesis, and fatty acid degradation pathways. This study provides valuable transcriptomic resources for the wax deficiency of cauliflower and lays a foundation for future research on genetic improvement and breeding strategies.</p>

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Transcriptome profiling of mRNA and lncRNA involved in wax biosynthesis in cauliflower

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年8月29日

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  • Kanghua Du,
  • Yirong Li,
  • Lingmin Wang,
  • Da Zhang,
  • Jixian Ma,
  • Lingfeng Bao,
  • Zhengfu Tang,
  • Jie Zhang,
  • Wanfu Mu,
  • Long Yang

摘要

The leaf wax layer in cauliflower (Brassica oleracea var. Botrytis L.) and other Brassica crops play an important role in environmental adaptation and defense. Here, high-throughput RNA sequencing was performed on leaves of a wax-deficient mutant type (WL) and its wild type (YL). A total of 43.13 Gb of raw RNA-seq data was obtained, of which 42.38 Gb of high-quality clean reads were retained after quality control. A total of 24,529 genes and 1,092 long non-coding RNA (lncRNA) were identified through transcriptome assembly and annotation. Functional enrichment analysis indicated that these mRNA and lncRNA are associated with biotic stress responses, lipid biosynthesis, and fatty acid degradation pathways. This study provides valuable transcriptomic resources for the wax deficiency of cauliflower and lays a foundation for future research on genetic improvement and breeding strategies.