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<p>Sarcosaprophagous insects play a pivotal role in forensic entomology, particularly in estimation of the postmortem interval (PMI). Among them, <i>Sarcophaga princeps</i> Wiedemann, 1830 (Diptera: Sarcophagidae), demonstrates distinct ecological traits such as larviposition and the ability to colonize buried remains. Even so, it has been hard to learn more about its molecular biology since a high-quality genome assembly is missing. This research uses PacBio HiFi, Illumina and Hi-C methods to produce a chromosome-level genome assembly for <i>S. princeps</i>. The resulting genome spans 628.56 Mb, with a scaffold N50 of 118.89 Mb, and 99.87% of the assembled sequence (627.74 Mb) successfully anchored to seven chromosomes. BUSCO analysis indicates that the genome is 99.10% complete, with 94.4% of genes present in single copy and 4.7% duplicated. Annotation of the genome identified 14,348 protein-coding genes and found that repetitive sequences occupy 333.04 Mb of the genome. The availability of this high-resolution genomic resource will greatly improve future research on the evolution, habitats and genomics of the Sarcophagidae family.</p>

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A chromosome-level genome assembly of Sarcophaga princeps Wiedemann, 1830 (Diptera: Sarcophagidae)

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年8月15日

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  • Liangliang Li,
  • Yinghui Wang,
  • Yingna Zhang,
  • Wenxuan Huang,
  • Shujin Li,
  • Yu Wang

摘要

Sarcosaprophagous insects play a pivotal role in forensic entomology, particularly in estimation of the postmortem interval (PMI). Among them, Sarcophaga princeps Wiedemann, 1830 (Diptera: Sarcophagidae), demonstrates distinct ecological traits such as larviposition and the ability to colonize buried remains. Even so, it has been hard to learn more about its molecular biology since a high-quality genome assembly is missing. This research uses PacBio HiFi, Illumina and Hi-C methods to produce a chromosome-level genome assembly for S. princeps. The resulting genome spans 628.56 Mb, with a scaffold N50 of 118.89 Mb, and 99.87% of the assembled sequence (627.74 Mb) successfully anchored to seven chromosomes. BUSCO analysis indicates that the genome is 99.10% complete, with 94.4% of genes present in single copy and 4.7% duplicated. Annotation of the genome identified 14,348 protein-coding genes and found that repetitive sequences occupy 333.04 Mb of the genome. The availability of this high-resolution genomic resource will greatly improve future research on the evolution, habitats and genomics of the Sarcophagidae family.