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<p>Yeasts are beneficial microorganisms for human society and are utilized for academic and industrial purposes. For academic purposes, <i>S. cerevisiae</i> is a well-investigated model for studying eukaryotic cellular processes. For industrial purposes, <i>S. pastorianus</i>, which has a hybrid genome of <i>S. cerevisiae</i> and <i>S. eubayanus</i>, has been served for lager beer production. During fermentation, <i>S. pastorianus</i> produces ~7% of EtOH, which induces plasma membrane (PM)/cell wall stress in yeast. Therefore, <i>S. pastorianus</i> may experience PM stress and adapt to the self-forming environment during fermentation. However, how yeast adapts to PM stress remains unclear. Here, we investigated the temporal cellular responses of <i>S. cerevisiae</i> and <i>S. pastorianus</i> during adaptation to PM stresses by time-resolved mRNA-seq analysis. Our data showed different transcriptional phenotypes between <i>S. cerevisiae</i> and <i>S. pastorianus</i> during adaptation. The results may reflect the distinct nature of the two yeasts that have evolved in different nutritional environments. The dataset presented here would provide a promising resource for studying the characteristic nature of these differentially domesticated yeasts upon PM stresses.</p>

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Time-resolved transcriptomics of S. cerevisiae and S. pastorianus in response to plasma membrane stresses

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年7月16日

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  • Yuta Yamazaki,
  • Fumihiko Omura,
  • Eiichiro Ono,
  • Nozomu Kamada,
  • Keiko Kono

摘要

Yeasts are beneficial microorganisms for human society and are utilized for academic and industrial purposes. For academic purposes, S. cerevisiae is a well-investigated model for studying eukaryotic cellular processes. For industrial purposes, S. pastorianus, which has a hybrid genome of S. cerevisiae and S. eubayanus, has been served for lager beer production. During fermentation, S. pastorianus produces ~7% of EtOH, which induces plasma membrane (PM)/cell wall stress in yeast. Therefore, S. pastorianus may experience PM stress and adapt to the self-forming environment during fermentation. However, how yeast adapts to PM stress remains unclear. Here, we investigated the temporal cellular responses of S. cerevisiae and S. pastorianus during adaptation to PM stresses by time-resolved mRNA-seq analysis. Our data showed different transcriptional phenotypes between S. cerevisiae and S. pastorianus during adaptation. The results may reflect the distinct nature of the two yeasts that have evolved in different nutritional environments. The dataset presented here would provide a promising resource for studying the characteristic nature of these differentially domesticated yeasts upon PM stresses.