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<p><i>Camellia nitidissima</i> is the model species of the <i>Camellia</i> sect. <i>Chrysantha</i> Chang, the only lineage within the genus <i>Camellia</i> known to produce golden-yellow flowers. This species holds high aesthetic, germplasm and medical value. Unfortunately, due to excessive collection and habitat loss, <i>C. nitidissima</i> is classified as a critically endangered plant. In this study, we assembled a telomere-to-telomere (T2T) genome of <i>C. nitidissima</i> by incorporating PacBio HiFi and Hi-C data. The assembled genome consisted of 15 pseudo-chromosomes, with a total size estimated to be 2.72 Gb. The GC content and repetitive sequences occupied 38.05% and 84.38% of the assembled genome, respectively. In total, 35,701 protein-coding genes were annotated. Multiple evaluation methods confirmed the contiguity (contig N50: 81.74 Mb), completeness (BUSCOs: 98.80%) and high LTR Assembly Index (LAI: 14.57) of the genome. This high-quality T2T genome will provide valuable insights into the genomic characteristics of <i>C. nitidissima</i> and facilitate conservation efforts as well as functional genomic studies in <i>Camellia</i> sect. <i>Chrysantha</i> species.</p>

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A telomere-to-telomere genome assembly of Camellia nitidissima

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年5月18日

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  • Xin-Feng Wang,
  • Tong-Jian Liu,
  • Tian Feng,
  • Hui-Run Huang,
  • Pu Zou,
  • Xiao Wei,
  • Xing Wu,
  • Sheng-Feng Chai,
  • Hai-Fei Yan

摘要

Camellia nitidissima is the model species of the Camellia sect. Chrysantha Chang, the only lineage within the genus Camellia known to produce golden-yellow flowers. This species holds high aesthetic, germplasm and medical value. Unfortunately, due to excessive collection and habitat loss, C. nitidissima is classified as a critically endangered plant. In this study, we assembled a telomere-to-telomere (T2T) genome of C. nitidissima by incorporating PacBio HiFi and Hi-C data. The assembled genome consisted of 15 pseudo-chromosomes, with a total size estimated to be 2.72 Gb. The GC content and repetitive sequences occupied 38.05% and 84.38% of the assembled genome, respectively. In total, 35,701 protein-coding genes were annotated. Multiple evaluation methods confirmed the contiguity (contig N50: 81.74 Mb), completeness (BUSCOs: 98.80%) and high LTR Assembly Index (LAI: 14.57) of the genome. This high-quality T2T genome will provide valuable insights into the genomic characteristics of C. nitidissima and facilitate conservation efforts as well as functional genomic studies in Camellia sect. Chrysantha species.