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<p><i>Fusarium neocosmosporiellum</i> causes alfalfa root rot, leading to significant economic losses. To explore the genetics of <i>F. neocosmosporiellum</i>, strain CA18-1 was isolated from infected alfalfa roots and subjected to whole-genome analysis. A scaffold-level reference genome of strain CA18-1 was assembled using both Nanopore long-read and Illumina short-read sequencing technologies. The assembled genome measured 63,424,297 base pairs (bp) in size, with a contig N50 of 6,480,858 bp and a contig N90 of 3,230,245 bp. A total of 10,486,459 bp repeat sequences and 28,006 protein-coding genes were predicted, of which 15,389 were functionally annotated. This study enhances our understanding of the genetic basis for <i>F. neocosmosporiellum</i>’s pathogenicity, providing a foundation for future studies on disease control and fungal evolution.</p>

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Scaffold-level genome assembly of Fusarium neocosmosporiellum strain CA18-1

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年5月8日

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  • Le Wang,
  • Ruifang Jia,
  • Jianjun Hao,
  • Zhengqiang Chen,
  • Xiaoluo Zhang,
  • Na Wang,
  • Shengze Wang,
  • Yuanyuan Zhang,
  • Kejian Lin

摘要

Fusarium neocosmosporiellum causes alfalfa root rot, leading to significant economic losses. To explore the genetics of F. neocosmosporiellum, strain CA18-1 was isolated from infected alfalfa roots and subjected to whole-genome analysis. A scaffold-level reference genome of strain CA18-1 was assembled using both Nanopore long-read and Illumina short-read sequencing technologies. The assembled genome measured 63,424,297 base pairs (bp) in size, with a contig N50 of 6,480,858 bp and a contig N90 of 3,230,245 bp. A total of 10,486,459 bp repeat sequences and 28,006 protein-coding genes were predicted, of which 15,389 were functionally annotated. This study enhances our understanding of the genetic basis for F. neocosmosporiellum’s pathogenicity, providing a foundation for future studies on disease control and fungal evolution.