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<p><i>Rosa laevigata</i> Michx., an endemic perennial herbaceous plant in China, possesses significant medicinal value in traditional herbal practices. However, the absence of a reference genome has hindered its development and utilization. In this study, we present a chromosome-level <Emphasis Type="BoldItalic">de novo</Emphasis> genome assembly of two haplotypes (Hap1 and Hap2) of <i>R. laevigata</i> by integration of Hifi long reads, BGI short reads, and Hi-C reads. The assembled Hap1 genome spans 493 Mb, Hap2 genome spans 479 Mb, and both of them were assigned to 7 chromosomes each. The mapping rate of BGI short reads to the two haplotypes genome is approximately 99.26% and 99.23%, and BUSCO assessment reveals that 98.6% and 98.7% of the genes are complete. Furthermore, we predicted 43,480 and 41,251 protein-coding genes in two haplotype genomes, respectively. The chromosome-level genome of <i>R. laevigata</i> enhances the genetic resources available for <i>Rosa</i> species and lays the groundwork for subsequent medicinal development.</p>

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Chromosomal level genome assembly of medicinal plant Rosa laevigata

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年4月30日

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  • Rongxiang Zhang,
  • Maohui Wang,
  • Guiyu Zhang,
  • Xiaoman Gao,
  • Niyan Xiang,
  • Shuwen Liu,
  • Yuemei Zhao,
  • Lijun Qin,
  • Tao Yuan

摘要

Rosa laevigata Michx., an endemic perennial herbaceous plant in China, possesses significant medicinal value in traditional herbal practices. However, the absence of a reference genome has hindered its development and utilization. In this study, we present a chromosome-level de novo genome assembly of two haplotypes (Hap1 and Hap2) of R. laevigata by integration of Hifi long reads, BGI short reads, and Hi-C reads. The assembled Hap1 genome spans 493 Mb, Hap2 genome spans 479 Mb, and both of them were assigned to 7 chromosomes each. The mapping rate of BGI short reads to the two haplotypes genome is approximately 99.26% and 99.23%, and BUSCO assessment reveals that 98.6% and 98.7% of the genes are complete. Furthermore, we predicted 43,480 and 41,251 protein-coding genes in two haplotype genomes, respectively. The chromosome-level genome of R. laevigata enhances the genetic resources available for Rosa species and lays the groundwork for subsequent medicinal development.