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<p><i>Trichogramma dendrolimi</i> is an essential egg parasitic wasp with a broad host range and has been widely used for controlling agricultural and forestry pests. Despite the availability of fragmented genomes of <i>Trichogramma</i>, a high-quality, chromosome-level genome reference is not yet available for this diverse genus. In this study, we assembled a high-quality chromosome-level genome of <i>T. dendrolimi</i> using PacBio CLR long sequencing and further scaffolded it with Hi-C technologies. The genome size was 216.24 Mb, featuring a N50 of 39.07 Mb, and 98.79% of scaffolds were anchored to five chromosomes. In addition, we annotated 12,902 protein-coding genes and 60.74 Mb repeat sequences for this genome assembly. In conclusion, our chromosome-level genome assembly provided important genomic resources that benefit the utilization of <i>Trichogramma</i> parasitoids in pest control.</p>

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Chromosomal-level genome assembly of Trichogramma dendrolimi (Trichogrammatidae: Hymenoptera)

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年4月21日

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  • Qianjin Dong,
  • Zhi Dong,
  • Sufang Ning,
  • Guiyun Li,
  • Wunan Che,
  • Gangqi Fang,
  • Shuai Zhan,
  • Jincheng Zhou,
  • Hui Dong

摘要

Trichogramma dendrolimi is an essential egg parasitic wasp with a broad host range and has been widely used for controlling agricultural and forestry pests. Despite the availability of fragmented genomes of Trichogramma, a high-quality, chromosome-level genome reference is not yet available for this diverse genus. In this study, we assembled a high-quality chromosome-level genome of T. dendrolimi using PacBio CLR long sequencing and further scaffolded it with Hi-C technologies. The genome size was 216.24 Mb, featuring a N50 of 39.07 Mb, and 98.79% of scaffolds were anchored to five chromosomes. In addition, we annotated 12,902 protein-coding genes and 60.74 Mb repeat sequences for this genome assembly. In conclusion, our chromosome-level genome assembly provided important genomic resources that benefit the utilization of Trichogramma parasitoids in pest control.