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<p><i>Trigonella corniculata</i> (L) L. or Nagauri pan /Kasuri methi, is an important spice crop with high nutraceutical potential. We report the <i>de novo</i> chromosome-scale assembly of <i>T. corniculata</i> genome using high coverage PacBio, Illumina and Hi-C reads. The assembly spans 798 Mb (Megabases) in 282 scaffolds with a scaffold N50 of 99.6 Mb. More than 98% of the sequence length is captured in eight different pseudomolecules with an average length of 98 Mb. A BUSCO score of over 97% is suggestive of the high degree of completeness and contiguity of the genome. A total of 64,801 protein-coding genes are predicted. Genome-wide Simple Sequence Repeats (99,149) have been identified and wet lab validated at forty-eight loci. The chromosome-scale genome assembly of <i>T. corniculata</i> and the SSR markers identified in this study will provide a strong foundation for future structural and functional genomics studies in <i>T. corniculata</i> and other fenugreek species.</p>

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Chromosome-scale genome assembly of Trigonella corniculata (L.)L. (Nagauri pan /Kasuri methi), an important spice

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年3月26日

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  • Ambika Baldev Gaikwad,
  • Sheel Yadav,
  • Ratna Kumari,
  • Wanchha Maurya,
  • Parimalan Rangan,
  • Rakesh Singh,
  • Gyanendra Pratap Singh

摘要

Trigonella corniculata (L) L. or Nagauri pan /Kasuri methi, is an important spice crop with high nutraceutical potential. We report the de novo chromosome-scale assembly of T. corniculata genome using high coverage PacBio, Illumina and Hi-C reads. The assembly spans 798 Mb (Megabases) in 282 scaffolds with a scaffold N50 of 99.6 Mb. More than 98% of the sequence length is captured in eight different pseudomolecules with an average length of 98 Mb. A BUSCO score of over 97% is suggestive of the high degree of completeness and contiguity of the genome. A total of 64,801 protein-coding genes are predicted. Genome-wide Simple Sequence Repeats (99,149) have been identified and wet lab validated at forty-eight loci. The chromosome-scale genome assembly of T. corniculata and the SSR markers identified in this study will provide a strong foundation for future structural and functional genomics studies in T. corniculata and other fenugreek species.