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<p>Crofton weed (<i>Ageratina adenophora</i>), a significant invasive species, extensively disrupts ecosystem stability, leading to considerable economic losses. However, genetic insights into its invasive mechanisms have been limited by a lack of genomic data. In this study, we present the successful <i>de novo</i> assembly of the triploid genome of <i>A. adenophora</i>, leveraging long-read PacBio Sequel, optical mapping, and Hi-C sequencing. Our assembly resolved into a haplotype-resolved genome comprising 51 chromosomes, with a total size of ~3.82 Gb and a scaffold N50 of 70.8 Mb. BUSCO analysis confirmed the completeness of 97.71% of genes. Genome annotation revealed 3.16 Gb (76.44%) of repetitive sequences and predicted 123,134 protein-coding genes, with 99.03% functionally annotated. The high-quality reference genome will provide valuable genomic resources for future studies on the evolutionary dynamics and invasive adaptations of <i>A. adenophora</i>.</p>

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Chromosome-level genome assembly of the crofton weed (Ageratina adenophora)

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年4月2日

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  • Fuyan Liu,
  • Lin Du,
  • Tingting Li,
  • Bo Liu,
  • Jianyang Guo,
  • Guifen Zhang,
  • Yibo Zhang,
  • Wanxue Liu,
  • Yan Pan,
  • Ying Zhang,
  • Hailing Wang,
  • Ruiying Li,
  • Weining Song,
  • Fanghao Wan

摘要

Crofton weed (Ageratina adenophora), a significant invasive species, extensively disrupts ecosystem stability, leading to considerable economic losses. However, genetic insights into its invasive mechanisms have been limited by a lack of genomic data. In this study, we present the successful de novo assembly of the triploid genome of A. adenophora, leveraging long-read PacBio Sequel, optical mapping, and Hi-C sequencing. Our assembly resolved into a haplotype-resolved genome comprising 51 chromosomes, with a total size of ~3.82 Gb and a scaffold N50 of 70.8 Mb. BUSCO analysis confirmed the completeness of 97.71% of genes. Genome annotation revealed 3.16 Gb (76.44%) of repetitive sequences and predicted 123,134 protein-coding genes, with 99.03% functionally annotated. The high-quality reference genome will provide valuable genomic resources for future studies on the evolutionary dynamics and invasive adaptations of A. adenophora.