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<p><i>Trilocha varians</i> is a member of the bombycid moths. Since <i>T. varians</i> has a considerably shorter generation period than the prevailing model species, <i>Bombyx mori</i>, this species would be a novel model insect in Lepidoptera. To facilitate further use of <i>T. varians</i>, we developed genome annotation information on the chromosome-scale assembly of <i>T. varians</i> previously published by our group. 9 RNA-seq datasets and 2 Iso-seq datasets were submitted for transcriptome-based gene prediction. As a result, 16,266 protein-coding genes were predicted on the latest genome assembly, and 98.6% of BUSCO sequences were present in our gene models. ATAC-seq was also conducted to determine chromatin accessibility across the genome. Finally, piRNA-targeted small RNA-seq revealed <i>T. varians</i> genome harbours 517 piRNA clusters (piCs). This information will encourage and facilitate potential users who plan to use this species.</p>

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Comprehensive genome annotation of Trilocha varians, a new model species of Lepidopteran insects

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年1月21日

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  • Jung Lee,
  • Toshiaki Fujimoto,
  • Katsushi Yamaguchi,
  • Shuji Shigenobu,
  • Ken Sahara,
  • Toru Shimada

摘要

Trilocha varians is a member of the bombycid moths. Since T. varians has a considerably shorter generation period than the prevailing model species, Bombyx mori, this species would be a novel model insect in Lepidoptera. To facilitate further use of T. varians, we developed genome annotation information on the chromosome-scale assembly of T. varians previously published by our group. 9 RNA-seq datasets and 2 Iso-seq datasets were submitted for transcriptome-based gene prediction. As a result, 16,266 protein-coding genes were predicted on the latest genome assembly, and 98.6% of BUSCO sequences were present in our gene models. ATAC-seq was also conducted to determine chromatin accessibility across the genome. Finally, piRNA-targeted small RNA-seq revealed T. varians genome harbours 517 piRNA clusters (piCs). This information will encourage and facilitate potential users who plan to use this species.