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<p>Recurrence of metabolic dysfunction-associated steatotic liver disease (MASLD) after liver transplantation (LT) is a continuing concern. The role of gut microbiome dysbiosis in MASLD initiation and progression has been well established. However, there is a lack of comprehensive gut microbiome shotgun sequence data for patients experiencing MASLD recurrence after LT. In this data descriptor, we describe a dataset of deep metagenomic sequences of a well-defined LT recipient population. Community-based analysis revealed a high abundance of <i>Akkermansia muciniphila</i>, consistently observed in most patient samples with a low (0–2) MASLD Activity Score (NAS). We constructed 357 metagenome-assembled genomes (MAGs), including 220 high-quality MAGs (&gt;90% completion). The abundance of different species of <i>Bacteroides</i> MAGs dominated in patient samples with NAS &gt; 5 (“definite MASH”). In contrast, the MAGs of <i>A. muciniphila</i>, <i>Akkermansia</i> sp., and <i>Blutia</i> sp. dominated in samples from patients without MASH (NAS = 0–2). In addition, the phylogenetic analysis of <i>A. muciniphila</i> and <i>Akkermansia</i> sp. MAGs identified two new phylogroups of <i>Akkermansia</i> that are distinct from the previously reported three phylogroups.</p>

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Deep sequencing-derived Metagenome Assembled Genomes from the gut microbiome of liver transplant patients

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2025年1月9日

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  • Goutam Banerjee,
  • Suraya Rahman Papri,
  • Hai Huang,
  • Sanjaya Kumar Satapathy,
  • Pratik Banerjee

摘要

Recurrence of metabolic dysfunction-associated steatotic liver disease (MASLD) after liver transplantation (LT) is a continuing concern. The role of gut microbiome dysbiosis in MASLD initiation and progression has been well established. However, there is a lack of comprehensive gut microbiome shotgun sequence data for patients experiencing MASLD recurrence after LT. In this data descriptor, we describe a dataset of deep metagenomic sequences of a well-defined LT recipient population. Community-based analysis revealed a high abundance of Akkermansia muciniphila, consistently observed in most patient samples with a low (0–2) MASLD Activity Score (NAS). We constructed 357 metagenome-assembled genomes (MAGs), including 220 high-quality MAGs (>90% completion). The abundance of different species of Bacteroides MAGs dominated in patient samples with NAS > 5 (“definite MASH”). In contrast, the MAGs of A. muciniphila, Akkermansia sp., and Blutia sp. dominated in samples from patients without MASH (NAS = 0–2). In addition, the phylogenetic analysis of A. muciniphila and Akkermansia sp. MAGs identified two new phylogroups of Akkermansia that are distinct from the previously reported three phylogroups.