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Chromosome-level assembly and evolution analysis of the Trichosanthes truncata genome

  • Ying Hu,
  • Xiaomei Wei,
  • Zhuannan Chu,
  • Fan Wei,
  • Yude Peng,
  • Baoyou Huang,
  • Ling Dong,
  • Kunhua Wei,
  • Weiwen Li

摘要

Trichosanthes truncata C. B. Clarke, an important medicinal plant, is a dioecious plant belonging to the Cucurbitaceae family. This study presents a chromosomal-level reference genome assembly for T. truncata. Through the integration of PacBio high-fidelity sequencing and high-throughput chromosome conformation capture technology, a final genome sequence of 637.41 Mb was assembled, with an N50 of 57.24 Mb and consisting of 11 pseudochromosomes. Additionally, 97.21 Mb of repetitive sequences and 36,172 protein-coding genes were annotated. This high-quality genome assembly is of utmost significance for studying the molecular mechanisms underlying the biosynthesis of bioactive compounds. Furthermore, this study provided valuable insights into plant comparative genomics research.