Scholarlink

<p>There is increasing interest in measuring the effect of microplastics and nanoplastics (MNPs) in the environment, but it is difficult to compare the results obtained in these studies due to variations in the extraction and characterization techniques, as well as the variability of the matrices analyzed. Here we provide a workflow consisting of three separate procedures for (1) preprocessing of different environmental samples, (2) methods for MNP extraction (four-step extraction method) and (3) techniques for qualitative and quantitative characterization of MNPs. The four-step extraction method (FSEM) involves predigestion, predensity separation, postdigestion and postdensity separation. This process has been optimized to maximize recovery (between 83.7% and 100% for polyethylene, polyethylene terephthalate, polypropylene, polystyrene and polymethyl methacrylate) and purity while minimizing artefactual changes to the particles. It is crucial to characterize the MNPs extracted using the FSEM to understand their chemical composition and other physicochemical properties such as quantity, particle size and morphology. We provide guidance on the use of different fit-for-purpose analytical technologies, including attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR), laser direct infrared spectroscopy (LDIR) and optical photothermal infrared microspectroscopy (O-PTIR). These techniques can be combined to characterize MNPs with particle sizes of 0.5–5,000 µm. We provide advice on how to optimize the sample preparation methods by adding or removing extraction steps based on the complexity of the matrix and purpose of the analysis. The suggested validation workflow also uses additional analytical techniques (for example, atomic force microscopy and flow cytometry) to evaluate efficiency, feasibility and reliability. We provide experimental detail for analysis using micro-FTIR, which can be used instead of LDIR and O-PTIR to characterize microplastics with particle sizes larger than 10 µm. Execution of this workflow takes 7–30 d and can be performed by researchers, technicians and students in the environmental field if they have access to the required equipment.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Extracting and characterizing microplastics and nanoplastics from environmental samples

  • 期刊论文

  • 发表日期：

    2025年11月14日

查看全文
  • Nana Liu,
  • Zifu Li,
  • Shikun Cheng,
  • Fazel Abdolahpur Monikh,
  • Ziwang Ye,
  • Tingting Ma,
  • Lei Zheng,
  • Xuemei Wang,
  • Bing-Jie Ni,
  • Zhijie Chen,
  • Baolong Jiang,
  • Peng Zhang,
  • Zhiling Guo,
  • Iseult Lynch

摘要

There is increasing interest in measuring the effect of microplastics and nanoplastics (MNPs) in the environment, but it is difficult to compare the results obtained in these studies due to variations in the extraction and characterization techniques, as well as the variability of the matrices analyzed. Here we provide a workflow consisting of three separate procedures for (1) preprocessing of different environmental samples, (2) methods for MNP extraction (four-step extraction method) and (3) techniques for qualitative and quantitative characterization of MNPs. The four-step extraction method (FSEM) involves predigestion, predensity separation, postdigestion and postdensity separation. This process has been optimized to maximize recovery (between 83.7% and 100% for polyethylene, polyethylene terephthalate, polypropylene, polystyrene and polymethyl methacrylate) and purity while minimizing artefactual changes to the particles. It is crucial to characterize the MNPs extracted using the FSEM to understand their chemical composition and other physicochemical properties such as quantity, particle size and morphology. We provide guidance on the use of different fit-for-purpose analytical technologies, including attenuated total reflection-Fourier transform infrared spectroscopy (ATR-FTIR), laser direct infrared spectroscopy (LDIR) and optical photothermal infrared microspectroscopy (O-PTIR). These techniques can be combined to characterize MNPs with particle sizes of 0.5–5,000 µm. We provide advice on how to optimize the sample preparation methods by adding or removing extraction steps based on the complexity of the matrix and purpose of the analysis. The suggested validation workflow also uses additional analytical techniques (for example, atomic force microscopy and flow cytometry) to evaluate efficiency, feasibility and reliability. We provide experimental detail for analysis using micro-FTIR, which can be used instead of LDIR and O-PTIR to characterize microplastics with particle sizes larger than 10 µm. Execution of this workflow takes 7–30 d and can be performed by researchers, technicians and students in the environmental field if they have access to the required equipment.