<p>Animals are motivated to seek information that does not influence reward outcomes, suggesting that information has intrinsic value. Here we have developed an odor-based information seeking task that reveals that mice choose to receive information even though it does not alter the reward outcome. Moreover, mice are willing to pay for information by sacrificing water reward, suggesting that information is of intrinsic value to a mouse. We used a microendoscope to reveal neural activity in the orbitofrontal cortex (OFC) while mice learned the information seeking task. We observed the emergence of distinct representations of odors predictive of information and odors predictive of water reward. A latent variable model recapitulated these different representations in the low-dimensional dynamics of OFC neuronal population activity. These data suggest that mice have evolved separate pathways to represent the intrinsic value of information and the extrinsic value of water reward. Thus, the desire to acquire knowledge is observed in mice and the value of this information is represented in the OFC. The mouse now provides a facile experimental system to study the representation of the value of information, a higher cognitive variable.</p>