Scholarlink

<p>Brain-resident macrophages, microglia, have been proposed to have an active role in synaptic refinement and maturation, influencing plasticity and circuit-level connectivity. Here we show that several neurodevelopmental processes previously attributed to microglia can proceed without them. Using a genetically modified mouse that lacks microglia (<i>Csf1r</i><sup>∆FIRE/∆FIRE</sup>), we find that intrinsic properties, synapse number and synaptic maturation are largely normal in the hippocampal CA1 region and somatosensory cortex at stages where microglia have been implicated. Seizure susceptibility and hippocampal-prefrontal cortex coherence in awake behaving animals, processes that are disrupted in mice deficient in microglia-enriched genes, are also normal. Similarly, eye-specific segregation of inputs into the lateral geniculate nucleus proceeds normally in the absence of microglia. Single-cell and single-nucleus transcriptomic analyses of neurons and astrocytes did not uncover any substantial perturbation caused by microglial absence. Thus, the brain possesses remarkable adaptability to execute developmental synaptic refinement, maturation and connectivity in the absence of microglia.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Typical development of synaptic and neuronal properties can proceed without microglia in the cortex and thalamus

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年1月6日

查看全文
  • Mary O’Keeffe,
  • Sam A. Booker,
  • Darren Walsh,
  • Mosi Li,
  • Chloe Henley,
  • Laura Simões de Oliveira,
  • Mingshan Liu,
  • Xingran Wang,
  • Maria Banqueri,
  • Katherine Ridley,
  • Kosala N. Dissanayake,
  • Cristina Martinez-Gonzalez,
  • Kirsty J. Craigie,
  • Deepali Vasoya,
  • Tom Leah,
  • Xin He,
  • David A. Hume,
  • Ian Duguid,
  • Matthew F. Nolan,
  • Jing Qiu,
  • David J. A. Wyllie,
  • Owen R. Dando,
  • Alfredo Gonzalez-Sulser,
  • Jian Gan,
  • Clare Pridans,
  • Peter C. Kind,
  • Giles E. Hardingham

摘要

Brain-resident macrophages, microglia, have been proposed to have an active role in synaptic refinement and maturation, influencing plasticity and circuit-level connectivity. Here we show that several neurodevelopmental processes previously attributed to microglia can proceed without them. Using a genetically modified mouse that lacks microglia (Csf1r∆FIRE/∆FIRE), we find that intrinsic properties, synapse number and synaptic maturation are largely normal in the hippocampal CA1 region and somatosensory cortex at stages where microglia have been implicated. Seizure susceptibility and hippocampal-prefrontal cortex coherence in awake behaving animals, processes that are disrupted in mice deficient in microglia-enriched genes, are also normal. Similarly, eye-specific segregation of inputs into the lateral geniculate nucleus proceeds normally in the absence of microglia. Single-cell and single-nucleus transcriptomic analyses of neurons and astrocytes did not uncover any substantial perturbation caused by microglial absence. Thus, the brain possesses remarkable adaptability to execute developmental synaptic refinement, maturation and connectivity in the absence of microglia.