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<p>Optimized sample preparation is essential for imaging biological macromolecules in their native state using single-particle cryo-electron microscopy (cryo-EM) or in situ cryo-electron tomography (cryo-ET). Here we present EasyGrid, a modular, automated platform designed to streamline and standardize cryo-EM/ET sample preparation. EasyGrid integrates in-line plasma treatment of the sample support, microfluidic dispensing, blot-less sample spreading, jet-based vitrification and grid quality control via light interferometry. We demonstrate its effectiveness by preparing grids for multiple purified macromolecular complexes and resolving their structures with cryo-EM. Additionally, EasyGrid achieves improved vitrification of large mammalian cells compared to conventional plunge-freezing. By enabling systematic and high-throughput optimization, EasyGrid provides a robust and time-saving solution for both structural and cellular cryo-EM applications.</p>

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EasyGrid: a versatile platform for automated cryo-EM sample preparation and quality control

  • 期刊论文

  • 出版模式：

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  • 发表日期：

    2026年6月23日

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  • Olivier Gemin,
  • Victor Armijo,
  • Léa Lecomte,
  • Michael Hons,
  • Thibault Deckers,
  • Caroline Bissardon,
  • Christopher Rossi,
  • Kévin Lauzier,
  • Robert Janocha,
  • Franck Felisaz,
  • Jérémy Sinoir,
  • Romain Linares,
  • Anastasiia Babenko,
  • Kirill Kovalev,
  • Irina Prokhorova,
  • Iskander Khusainov,
  • Claudia Schreiner,
  • Olga Kolesnikova,
  • Veijo T. Salo,
  • Sarah Schneider,
  • Matthew W. Bowler,
  • Georg Wolff,
  • Wojciech P. Galej,
  • Julia Mahamid,
  • Christoph W. Müller,
  • Kristina Djinovic Carugo,
  • Sebastian Eustermann,
  • Simone Mattei,
  • Florent Cipriani,
  • Gergely Papp

摘要

Optimized sample preparation is essential for imaging biological macromolecules in their native state using single-particle cryo-electron microscopy (cryo-EM) or in situ cryo-electron tomography (cryo-ET). Here we present EasyGrid, a modular, automated platform designed to streamline and standardize cryo-EM/ET sample preparation. EasyGrid integrates in-line plasma treatment of the sample support, microfluidic dispensing, blot-less sample spreading, jet-based vitrification and grid quality control via light interferometry. We demonstrate its effectiveness by preparing grids for multiple purified macromolecular complexes and resolving their structures with cryo-EM. Additionally, EasyGrid achieves improved vitrification of large mammalian cells compared to conventional plunge-freezing. By enabling systematic and high-throughput optimization, EasyGrid provides a robust and time-saving solution for both structural and cellular cryo-EM applications.