Scholarlink

<p>The formation of organized optical states in multidimensional systems is crucial for understanding light–matter interaction and advancing light-shaping technologies. Here we report the observation of a self-localized, ultrafast pencil beam near the critical power in a standard multimode fiber. We demonstrate that self-focusing, traditionally considered detrimental, facilitates a nonlinear spatiotemporal localized state with a sidelobe-suppressed Bessel-like profile and markedly improved stability. Generated simply by an on-axis Gaussian launch, this beam is readily integrated into standard multiphoton microscopes. We applied this self-localized beam to two-photon imaging of mouse enteric nervous systems, where it outperformed conventional Bessel beams through reduced sidelobes and enhanced aberration resilience. Lastly, we monitored transferrin uptake dynamics in a live human blood–brain barrier model using minute-resolved three-dimensional scans, revealing spatiotemporal heterogeneity across different cell types. Our findings offer a robust approach for generating ultrafast pencil beams, enabling high-throughput three-dimensional biosystem imaging to elucidate biological transport pathways.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

Self-localized ultrafast pencil beam for volumetric multiphoton imaging

  • 期刊论文

  • 发表日期：

    2026年4月27日

查看全文
  • Honghao Cao,
  • Sarah Spitz,
  • Li-Yu Yu,
  • Kunzan Liu,
  • Zhengyu Zhang,
  • Federico Presutti,
  • Francesca Michela Pramotton,
  • Subhash Kulkarni,
  • Roger D. Kamm,
  • Sixian You

摘要

The formation of organized optical states in multidimensional systems is crucial for understanding light–matter interaction and advancing light-shaping technologies. Here we report the observation of a self-localized, ultrafast pencil beam near the critical power in a standard multimode fiber. We demonstrate that self-focusing, traditionally considered detrimental, facilitates a nonlinear spatiotemporal localized state with a sidelobe-suppressed Bessel-like profile and markedly improved stability. Generated simply by an on-axis Gaussian launch, this beam is readily integrated into standard multiphoton microscopes. We applied this self-localized beam to two-photon imaging of mouse enteric nervous systems, where it outperformed conventional Bessel beams through reduced sidelobes and enhanced aberration resilience. Lastly, we monitored transferrin uptake dynamics in a live human blood–brain barrier model using minute-resolved three-dimensional scans, revealing spatiotemporal heterogeneity across different cell types. Our findings offer a robust approach for generating ultrafast pencil beams, enabling high-throughput three-dimensional biosystem imaging to elucidate biological transport pathways.