Scholarlink

<p>Monitoring neuronal activity at large scale and high spatiotemporal resolution is crucial for understanding information processing within the brain. Here we optimized a kilohertz-frame-rate two-photon fluorescence microscope with an all-optical megahertz line-scan rate to achieve ultrafast imaging across large areas and volumes at subcellular resolution. Applying this technique to in vivo voltage and calcium imaging, we demonstrated simultaneous recording of voltage activity over 200 neurons and calcium activity over 14,000 neurons from the mouse visual cortex, as well as volumetric calcium imaging of the larval zebrafish brain.</p>

错误：搜索内容不能为空，请输入英文关键词
错误：关键词超出字数限制，请精简
高级检索

FACED 2.0 enables large-scale voltage and calcium imaging in vivo

  • 期刊论文

  • 出版模式：

    开放获取

  • 发表日期：

    2025年12月9日

查看全文
  • Jian Zhong,
  • Ryan G. Natan,
  • Qinrong Zhang,
  • Justin S. J. Wong,
  • Christoph Miehl,
  • Krishnashish Bose,
  • Xiaoyu Lu,
  • François St-Pierre,
  • Su Guo,
  • Brent Doiron,
  • Kevin K. Tsia,
  • Na Ji

摘要

Monitoring neuronal activity at large scale and high spatiotemporal resolution is crucial for understanding information processing within the brain. Here we optimized a kilohertz-frame-rate two-photon fluorescence microscope with an all-optical megahertz line-scan rate to achieve ultrafast imaging across large areas and volumes at subcellular resolution. Applying this technique to in vivo voltage and calcium imaging, we demonstrated simultaneous recording of voltage activity over 200 neurons and calcium activity over 14,000 neurons from the mouse visual cortex, as well as volumetric calcium imaging of the larval zebrafish brain.