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<p>Advanced brain-wide mapping is critical for addressing complex questions in neuroscience. However, current imaging methods are limited by throughput, resolution and signal-to-noise ratio, constraining their broader applicability. Here, we present confocal Airy beam integrated with single-photon oblique light-sheet tomography (CAB-OLST): a system that integrates single-photon excitation with a scanned Airy beam light sheet, virtual slit detection and automated mechanical sectioning. CAB-OLST enables high-throughput, high-resolution and high-signal-to-noise ratio volumetric imaging, achieving an optical resolution of 0.77 μm × 0.49 μm × 2.61 μm. This allows for mouse brain-wide cell type distribution mapping at a voxel size of 0.37 μm × 0.37 μm × 1.77 μm in 10 h and single-neuron projectome imaging with a voxel size of 0.26 μm × 0.26 μm × 1.06 μm over 58 h. Compared to existing light-sheet and point-scanning systems, CAB-OLST provides a scalable and robust platform for comprehensive neuronal morphology reconstruction and high-precision cell atlas generation.</p>

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Confocal Airy beam oblique light-sheet tomography for brain-wide cell type distribution and morphology

  • 期刊论文

  • 发表日期：

    2025年11月13日

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  • Xiaoli Qi,
  • Rodrigo Muñoz-Castañeda,
  • Yuanlei Yue,
  • Sheng Xiao,
  • Liya Ding,
  • Arun Narasimhan,
  • Xin Chen,
  • Wei Wang,
  • Yuanyuan Li,
  • Corey Elowsky,
  • Jason Palmer,
  • Rhonda Drewes,
  • Jianjun Sun,
  • Jinglei Wu,
  • Lei Qu,
  • Zhuhao Wu,
  • Hanchuan Peng,
  • Pavel Osten

摘要

Advanced brain-wide mapping is critical for addressing complex questions in neuroscience. However, current imaging methods are limited by throughput, resolution and signal-to-noise ratio, constraining their broader applicability. Here, we present confocal Airy beam integrated with single-photon oblique light-sheet tomography (CAB-OLST): a system that integrates single-photon excitation with a scanned Airy beam light sheet, virtual slit detection and automated mechanical sectioning. CAB-OLST enables high-throughput, high-resolution and high-signal-to-noise ratio volumetric imaging, achieving an optical resolution of 0.77 μm × 0.49 μm × 2.61 μm. This allows for mouse brain-wide cell type distribution mapping at a voxel size of 0.37 μm × 0.37 μm × 1.77 μm in 10 h and single-neuron projectome imaging with a voxel size of 0.26 μm × 0.26 μm × 1.06 μm over 58 h. Compared to existing light-sheet and point-scanning systems, CAB-OLST provides a scalable and robust platform for comprehensive neuronal morphology reconstruction and high-precision cell atlas generation.